LENTROPIN, A PROTEIN THAT CONTROLS LENS FIBER FORMATION, IS RELATED FUNCTIONALLY AND IMMUNOLOGICALLY TO THE INSULIN-LIKE GROWTH-FACTORS
LENTROPIN, A PROTEIN THAT CONTROLS LENS FIBER FORMATION, IS RELATED FUNCTIONALLY AND IMMUNOLOGICALLY TO THE INSULIN-LIKE GROWTH-FACTORS
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DOI：
10.1073/pnas.84.8.2327
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发表时间：
1987-04-01
影响因子：
11.1
通讯作者：
ZELENKA, PS
中科院分区：
文献类型：
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作者：
BEEBE, DC;SILVER, MH;ZELENKA, PS
Lentropin是一种存在于眼玻璃体液中的因子，在体外刺激鸡胚透镜上皮细胞分化为透镜纤维。伦托品已部分纯化，但尚未分离出足够的量或纯度以与其他生长和分化因子直接比较。先前的研究已经表明，胰岛素和胎牛血清与lentropin共享刺激培养的上皮细胞形成透镜纤维的能力。在本研究中，测定了一些激素和生长因子的lentropin活性。在这些测试中，唯一具有这种活性的物质是胰岛素样生长因子（IGFs）生长调节素C（Sm-C/IGF-I）和增殖刺激活性（MSA/IGF-II）。Sm-C/IGF-I在促进纤维细胞形成方面的效力比胰岛素或MSA/IGF-II强约30倍。人Sm-C/IGF-I单克隆抗体抑制纯化Sm-C/IGF-I，胎牛血清，鸡玻璃体液刺激纤维细胞分化在体外。该抗体已显示不与胰岛素交叉反应，并且不阻断胰岛素刺激的透镜纤维形成。这些发现表明，lentropin与IGFs相关，这些因子除了具有更好的刺激细胞分裂的能力外，还可能在控制细胞分化中发挥重要作用。
Lentropin, a factor present in the vitreous humor of the eye, stimulates lens fiber differentiation from chicken embryo lens epithelial cells in vitro. Lentropin has been partially purified but has not been isolated in sufficient quantity or purity for direct comparison with other growth and differentiation factors. Previous studies have shown that insulin and fetal bovine serum share with lentropin the ability to stimulate lens fiber formation from cultured epithelial cells. In the present study, a number of hormones and growth factors were assayed for lentropin activity. Of those tested, the only substances that had this activity were the insulin-like growth factors (IGFs) somatomedin C (Sm-C/IGF-I) and multiplication-stimulating activity (MSA/IGF-II). Sm-C/IGF-I was approximately 30 times more potent than insulin or MSA/IGF-II in promoting fiber cell formation. A monoclonal antibody to human Sm-C/IGF-I inhibited purified Sm-C/IGF-I, fetal bovine serum, and chicken vitreous humor from stimulating fiber cell differentiation in vitro. This antibody has been shown not to crossreact with insulin and did not block insulin-stimulated lens fiber formation. These findings indicate that lentropin is related to the IGFs and that these factors may play important roles in controlling cell differentiation, in addition to their better-known ability to stimulate cell division.