MONONUCLEAR PHAGOCYTES OF BLOOD AND BONE-MARROW - COMPARATIVE ROLES AS VIRAL RESERVOIRS IN HUMAN IMMUNODEFICIENCY VIRUS TYPE-1 INFECTIONS
MONONUCLEAR PHAGOCYTES OF BLOOD AND BONE-MARROW - COMPARATIVE ROLES AS VIRAL RESERVOIRS IN HUMAN IMMUNODEFICIENCY VIRUS TYPE-1 INFECTIONS
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DOI：
10.1073/pnas.86.2.675
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发表时间：
1989-01-01
影响因子：
11.1
通讯作者：
COHN, ZA
中科院分区：
文献类型：
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作者：
MCELRATH, MJ;PRUETT, JE;COHN, ZA
我们研究了人类免疫缺陷病毒（HIV）的生产培养的单核细胞从36个血清阳性的同性恋男性。使用HIV p24抗原ELISA检测，在54%的无症状，71%的获得性免疫缺陷综合征（AIDS）相关的复杂，和100%的艾滋病患者的血液单核细胞中检测到生产。当外周血单个核细胞被分离成单核细胞和CD 4 + T细胞时，我们发现在三个临床阶段中，CD 4 + T细胞组分优先被感染。从血液单核细胞衍生的巨噬细胞中分离HIV的能力在三个阶段相似（24-33%），需要与植物血凝素刺激的淋巴母细胞共培养。同时培养的骨髓和血液单核细胞在13个个体中从两种来源产生病毒。同样，病毒的主要来源是含有CD 4 + T细胞的非粘附骨髓单核细胞，而不是粘附单核细胞富集部分。我们的结论是，血液单核细胞产生生产病毒更容易随着疾病的进展，并检测到感染与CD 4 + T细胞富集的馏分。在我们的大样本患者中，仅在一小部分中检测到单核细胞感染，表明这种细胞类型既不是HIV感染的主要也不是唯一的储存库。
We examined human immunodeficiency virus (HIV) production in cultured mononuclear cells from 36 seropositive homosexual males. Production was detected by using an HIV p24 antigen ELISA assay in blood mononuclear cells in 54% of asymptomatic, 71% of acquired immunodeficiency syndrome (AIDS)-related complex, and 100% of AIDS patients. When the peripheral blood mononuclear cells were separated into monocytes and CD4+ T cells, we found that the CD4+ T-cell fraction was preferentially infected in the three clinical stages. The ability to isolate HIV from blood monocyte-derived macrophages was similar in the three stages (24-33%) and required coculture with phytohemagglutinin-stimulated lymphoblasts. Bone marrow and blood mononuclear cells cultured simultaneously yielded virus from both sources in 13 individuals. Again the prime source of virus was the nonadherent bone marrow mononuclear cells, which contained CD4+ T cells, and not the adherent monocyte-enriched fraction. We conclude that blood mononuclear cells yield productive virus more readily as disease progresses and that infection is detected in association with CD4+ T-cell-enriched fractions. In our large sample of patients, monocyte infection was detected in only a small fraction, suggesting that this cell type is neither a primary nor an exclusive reservoir of HIV infection.