Young adult patients with squamous cell carcinoma of the tongue strongly express p16 without human papillomavirus infection
Young adult patients with squamous cell carcinoma of the tongue strongly express p16 without human papillomavirus infection
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未感染人乳头瘤病毒的年轻舌鳞状细胞癌患者强表达 p16
DOI：
10.1080/00016489.2018.1541506
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发表时间：
2019
影响因子：
1.4
通讯作者：
Sato Yasuharu
中科院分区：
文献类型：
--
作者：
Tachibana Tomoyasu;Orita Yorihisa;Gion Yuka;Miki Kentaro;Ikegami Kana;Marunaka Hidenori;Makino Takuma;Akagi Yusuke;Akisada Naoki;Tsumura Munechika;Ito Toshihiro;Yoshino Tadashi;Nishizaki Kazunori;Sato Yasuharu
背景：长期吸烟和饮酒是已知的舌癌（TC）的发病原因。目的：探讨TC与人乳头瘤病毒（HPV）感染的关系。材料与方法：对86例TC患者（<40岁12例，≥40岁74例）的临床资料和手术标本进行分析。强的细胞核和细胞质p16染色被认为是阳性的。采用聚合酶链反应（PCR）检测HPV DNA（高危亚型：16、18、31、33、35、52 b和58;低危亚型：6和11）。9例（10.5%）患者检测到HPV DNA（高危亚型，n= 2;低危亚型，n= 7）。p16强表达在年轻人中比在老年人中更常见（33.3%vs.8.1%;p= .045）。p16染色与HPV DNA检测无相关性（相关系数0.113;p= 0.300）。结论和意义：TC中p16表达与HPV感染无关，提示其可能由不同的机制引起。
Background:Long-term smoking and drinking are known to contribute to the onset of tongue cancer (TC). However, the increasing incidence of TC in younger adults has been suggested to be associated with other factors.Objectives:The present study investigated the relationship between TC and human papillomavirus (HPV) infection.Material and methods:Clinical records and surgically resected specimens from 86 patients (<40-years-old,n= 12; ≥40-years-old,n= 74) with TC were analyzed. Strong nuclear and cytoplasmic p16 staining was considered positive. HPV DNA (high-risk subtypes: 16, 18, 31, 33, 35, 52b, and 58; low-risk subtypes: 6 and 11) was detected using consensus primer-mediated polymerase chain reaction.Results:Strong p16 expression was observed in 10 (11.6%) patients. HPV DNA was detected in 9 (10.5%) patients (high-risk subtypes,n= 2; low-risk subtypes,n= 7). Strong p16 expression was observed more frequently among younger adults than among older adults (33.3%vs.8.1%;p= .045). p16 staining did not correlate with the detection of HPV DNA (correlation coefficient, 0.113;p= .300).Conclusions and significance:In TC, p16 expression was not associated with HPV infection, suggesting that it may be caused by a different mechanism.