Mammalian chromosomes in vitro. IX. On genetic polymorphism in cell populations.
Mammalian chromosomes in vitro. IX. On genetic polymorphism in cell populations.
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体外哺乳动物染色体。
DOI：
10.1093/jnci/21.3.437
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发表时间：
1958
期刊：
影响因子：
--
通讯作者：
Oleta Klatt
中科院分区：
文献类型：
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作者：
T. Hsu;Oleta Klatt
研究了小鼠来源的五种细胞株 MC3、CF1-C-9、NCTC 2071（NCTC 克隆 929，菌株 L）、L-P55 和 L-P55-N2 的染色体构成。最后 3 个菌株是 NCTC 克隆 929 的亚系（菌株 L）。它们都是异倍体。除了染色体数目的变化外，每个菌株还拥有10至20条中着丝粒和一些亚端着丝粒染色体。每个菌株都有其特征性的核型并且可以通过细胞学方法进行鉴定。 L-P55和L-P55-N2菌株的细胞含有1或2条长双着丝粒染色体。然而，L-P55 菌株中的一小部分细胞不含这种元素。该双着丝粒染色体可能与 Fedoroff 在加拿大萨斯卡通建立的 L2 菌株中经常出现的双着丝粒染色体相同。在每个菌株中，记录了染色体数量以及染色体类型组合的相当大的变化。因此，每个菌株的基因组成都是异质的。可以想象，遗传多态性在其他细胞株群体中也很普遍。它为群体提供了不同的基因型，以适应可能施加于他们的各种逆向选择力量。
Five cell strains MC3, CF1-C-9, NCTC 2071 (NCTC clone 929, strain L), L-P55, and L-P55-N2, all of murine origin, were studied with respect to their chromosomal constitution. The last 3 strains were sublines of NCTC clone 929 (strain L). All of them were heteroploid. Besides the changes in chromosome number, each strain possessed from 10 to 20 metacentrics and a few subtelocentric chromosomes. Each strain had its characteristic karyotype and was cytologically identifiable. Cells of strains L-P55 and L-P55-N2 contained 1 or 2 long dicentric chromosomes. However, a small proportion of the cells in strain L-P55 was void of this element. This dicentric chromosome was probably identical to the dicentric that occurred frequently in strain L2established by Fedoroff in Saskatoon, Canada. Within each strain considerable variations of chromosome number as well as combination of chromosome types were recorded. Therefore, each strain is heterogeneous in its genetic make-up. Genetic polymorphism conceivably prevails also in populations of other cell strains. It provides different genotypes for the populations to adjust themselves to various adverse selection forces that may be applied to them.