Vitamin D stimulates prolactin synthesis by GH4C1 cells incubated in chemically defined medium.
Vitamin D stimulates prolactin synthesis by GH4C1 cells incubated in chemically defined medium.
复制标题
维生素 D 刺激在化学成分确定的培养基中孵育的 GH4C1 细胞合成催乳素。
作者：
J. Wark;A. Tashjian
GH4C1 cells were evaluated as a model system for the investigation of the cellular and molecular mode of action of vitamin D. When cells were incubated in hormone-free, chemically defined medium, the metabolite 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) potently stimulated PRL synthesis (ED50 = 2 x 10(-10) M) with minimal effects on GH synthesis. The stimulation of PRL synthesis was vitamin D metabolite specific. The effect of 1,25-(OH)2D3 was dependent on the addition of extracellular calcium in the concentration range up to 0.1 mM. These results indicate the potential usefulness of this experimental system for the study of vitamin D action.
