Rapid Multiplex Small DNA Sequencing on the MinION Nanopore Sequencing Platform.
Rapid Multiplex Small DNA Sequencing on the MinION Nanopore Sequencing Platform.
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DOI：
10.1534/g3.118.200087
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发表时间：
2018-05-04
期刊：
影响因子：
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通讯作者：
Williams Z
中科院分区：
文献类型：
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作者：
Wei S;Weiss ZR;Williams Z
短DNA片段的实时测序具有广泛的临床和研究应用，包括筛选突变、靶序列和非整倍体。我们最近展示了Minion，一个USB驱动器大小的基于纳米孔的DNA测序设备，可以用于短读DNA测序。本研究提出了一种超快速多重文库制备和测序方法，并应用于正常二倍体和非整倍体样本的基因组DNA的快速检测，包括文库制备和测序。这种新的方法显示了巨大的前景，作为一种临床诊断测试需要快速短读DNA测序的应用。
Real-time sequencing of short DNA reads has a wide variety of clinical and research applications including screening for mutations, target sequences and aneuploidy. We recently demonstrated that MinION, a nanopore-based DNA sequencing device the size of a USB drive, could be used for short-read DNA sequencing. In this study, an ultra-rapid multiplex library preparation and sequencing method for the MinION is presented and applied to accurately test normal diploid and aneuploidy samples’ genomic DNA in under three hours, including library preparation and sequencing. This novel method shows great promise as a clinical diagnostic test for applications requiring rapid short-read DNA sequencing.