Fast detection of volatile organic compounds from Staphylococcal blood cultures by CDI-MS
Fast detection of volatile organic compounds from Staphylococcal blood cultures by CDI-MS
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利用 CDI-MS 快速检测葡萄球菌血培养物中的挥发性有机化合物
DOI：
10.1039/c7ra01815k
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发表时间：
2017-01-01
期刊：
影响因子：
3.9
通讯作者：
Hu, Longhua
中科院分区：
文献类型：
--
作者：
Hang, Yaping;Chingin, Konstantin;Hu, Longhua
葡萄球菌菌血症的快速识别是临床研究中的一个严峻挑战。在这里，我们报告了直接分析的分子挥发性有机化合物（VOCs）的血培养35例患者发出的怀疑葡萄球菌菌血症和31例确诊为葡萄球菌菌血症的指纹，通过大气压电晕放电电离质谱（CDI-MS）的存在下，四种常见的葡萄球菌（金黄色葡萄球菌，S。hominis，S. haemolyticus和S.表皮），其在统计学上解释了ca. 90%的人葡萄球菌菌血症病例。所有感染的血培养物在孵育3 - 16 h后均显示出特征性和明显不同的MS模式，特异于四种葡萄球菌中的一种。将技术重复培养2 - 3天，用于使用当前常规菌血症检测进行参考诊断。我们的6个月医院研究的结果证实，在m/z 87（异丁酸）和m/z 101（异戊酸）信号的峰是专门存在于葡萄球菌，并表明CDI-MS的快速，可靠和成本效益的诊断葡萄球菌菌血症在人类中的高度适用性。
Rapid recognition of Staphylococcal bacteremia in humans is a serious challenge in clinical research. Here we report on direct analysis of molecular volatile organic compounds (VOCs) of blood cultures emitted by 35 patients with a suspicion of Staphylococcal bacteremia and 31 patients positively diagnosed with Staphylococcal bacteremia were fingerprinted by atmospheric corona discharge ionization mass spectrometry (CDI-MS) for the presence of four common Staphylococci (Staphylococcus aureus, S. hominis, S. haemolyticus, and S. epidermidis) which statistically account for ca. 90% of cases of Staphylococcal bacteremia in humans. All the infected blood cultures revealed characteristic and clearly distinct MS patterns specific to the presence of one of the four Staphylococci after an incubation time of 3–16 h. Technical replicates were incubated over 2–3 days for reference diagnosis using current conventional bacteremia detection. The results of our 6 month hospital study confirm peaks at m/z 87 (isobutyric acid) and m/z 101 (isovaleric acid) signals were specifically present in Staphylococci, and indicate that high suitability of CDI-MS for rapid, reliable and cost-efficient diagnosis of Staphylococcal bacteremia in humans.