Plasma free amino acid profiling of esophageal cancer using high-performance liquid chromatography spectroscopy
Plasma free amino acid profiling of esophageal cancer using high-performance liquid chromatography spectroscopy
复制标题
使用高效液相色谱法分析食管癌的血浆游离氨基酸
DOI：
10.3748/wjg.v20.i26.8653
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发表时间：
2014-07-14
影响因子：
4.3
通讯作者：
Sheyhidin, Ilyar
中科院分区：
文献类型：
--
作者：
Ma, Hong;Hasim, Ayshamgul;Sheyhidin, Ilyar
目的：目的：研究不同病理阶段食管鳞癌（ESCC）患者和健康对照者血浆游离氨基酸（PFAA）谱，方法：采用高效液相色谱法（HPLC）分析51例ESCC患者和60例健康对照者血浆中PFAA谱。食管鳞癌中分化程度低者24例，淋巴结转移者17例，临床分期> Ib 2者36例。偏最小二乘判别分析表明，PFAA的代谢模式，使ESCC患者和对照之间的歧视，学生t检验被应用到评估PFAA浓度之间的显着差异，两组之间的groups.RESULTS：有显着差异PFAA档案之间的控制和ESCC患者。与健康对照组相比，食管鳞癌患者血清中Asp、Glu、Gly、His、Thr、Tau、Ala、Met、Ile、Leu、Phe水平降低，Cys水平升高。食管鳞癌患者的PFAA谱与临床病理特征之间存在很强的相关性。许多PFAA的水平（即，Glu、Asp、Ser、Gly、Tau、Ala、Tyr、瓦尔、Ile、Leu）与食管鳞癌病理分级、淋巴结转移、临床分期有关。ESCC患者和对照受试者之间实现了很好的歧视，通过多变量建模的血浆profiles.CONCLUSION：基于HPLC的血浆分析被证明是一种有效的方法来区分ESCC患者和对照。PFAA谱可能对食管鳞癌的筛查和诊断有潜在价值。(C)2014百世登出版集团股份有限公司All rights reserved.
AIM: To perform plasma free amino acid (PFAA) profiling of esophageal squamous cell carcinoma (ESCC) patients at different pathological stages and healthy subjects.METHODS: Plasma samples from ESCC patients (n = 51) and healthy control adults (n = 60) were analyzed by high-performance liquid chromatography (HPLC). The ESCC patients included moderate/poorly-differentiation (n = 24), lymph node metastasis (n = 17) and clinical stage > Ib2 (n = 36). Partial least squares discriminant analysis was performed to demonstrate that the PFAA metabolic patterns enabled discrimination between ESCC patients and controls, and the Student t test was applied to assess significant differences in PFAA concentrations between the two groups.RESULTS: There were significant differences in the PFAA profiles between controls and ESCC patients. Compared with healthy controls, the levels of Asp, Glu, Gly, His, Thr, Tau, Ala, Met, Ile, Leu, and Phe were decreased in ESCC patients, but Cys was increased. There exists a strong correlation between PFAA profiles and clinicopathological characteristics in ESCC patients. The levels of many PFAAs (i.e., Glu, Asp, Ser, Gly, Tau, Ala, Tyr, Val, Ile, and Leu) were related to pathological grading, lymph node metastasis, and ESCC clinical stage. Very good discrimination between ESCC patients and control subjects was achieved by multivariate modeling of plasma profiles.CONCLUSION: HPLC-based plasma profiling analysis was shown to be an effective approach to differentiate between ESCC patients and controls. PFAA profiles may have potential value for screening or diagnosing ESCC. (C) 2014 Baishideng Publishing Group Inc. All rights reserved.