Comparison of nucleotide sequences of recent and previous lineages of peste-des-petits-ruminants viruses of sheep and goats in Nigeria
Comparison of nucleotide sequences of recent and previous lineages of peste-des-petits-ruminants viruses of sheep and goats in Nigeria
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DOI：
10.4102/ojvr.v83i1.1163
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发表时间：
2016-01-01
影响因子：
2.7
通讯作者：
van Vuuren, Moritz
中科院分区：
文献类型：
--
作者：
Mantip, Samuel;Quan, Melvyn;van Vuuren, Moritz
小反刍兽疫病毒 (PPRV) 是一种高度传染性、致命性和经济重要性的小反刍动物病毒性疾病，目前仍呈地方性流行，并影响世界流行地区绵羊和山羊的生产。这项研究的目的是描述该国境内的病毒株。这是通过从尼日利亚各个农业生态区的绵羊和山羊身上收集组织和拭子样本来进行的。通过核蛋白 (N) 基因测序确定已存档的 PPRV 毒株或分离株以及最近流行并引起爆发的毒株或分离株的系统发育。从 18 个州（即访问的 6 个农业生态区各 3 个州）中随机采集了 20 份来自表面健康和患病绵羊和山羊的组织和拭子样本。总共采集了360个样本。 360份样本中共有35份（9.7%）通过逆转录酶-聚合酶链式反应检测呈阳性，其中25份来自眼鼻拭子，10份来自组织样本。使用简约系统发育分析 (PAUP) 进行的邻接系统发育分析确定了四个不同的谱系，即谱系 I、II、III 和 IV。有趣的是，本研究中描述的尼日利亚菌株分为两个独立的主要谱系，即谱系 II 和 IV。来自索科托、奥约、高原和翁多州的毒株根据 PPRV 的历史分布与尼日利亚 75/1 毒株归为 II 系，而来自索科托、奥约、高原、阿夸伊博姆、阿达马瓦、卡杜纳、拉各斯、包奇、尼日尔和卡诺州的其他毒株则与东非和亚洲毒株归为 IV 系。这一发现证实了 PPRV 谱系 II 和 IV 株均在尼日利亚流行。此前，该国仅发现了II系菌株。
Peste-des-petits-ruminants virus (PPRV) is a highly contagious, fatal and economically important viral disease of small ruminants that is still endemic and militates against the production of sheep and goats in endemic areas of the world. The aim of this study was to describe the viral strains within the country. This was carried out by collecting tissue and swab samples from sheep and goats in various agro-ecological zones of Nigeria. The phylogeny of archived PPRV strains or isolates and those circulating and causing recent outbreaks was determined by sequencing of the nucleoprotein (N)-gene. Twenty tissue and swab samples from apparently healthy and sick sheep and goats were collected randomly from 18 states, namely 3 states in each of the 6 agro-ecological zones visited. A total of 360 samples were collected. A total of 35 samples of 360 (9.7%) tested positive by reverse transcriptase-polymerase chain reaction, of which 25 were from oculo-nasal swabs and 10 were from tissue samples. Neighbour-joining phylogenetic analysis using Phylogenetic Analysis Using Parsimony (PAUP) identified four different lineages, that is, lineages I, II, III and IV. Interestingly, the Nigerian strains described in this study grouped in two separate major lineages, that is, lineages II and IV. Strains from Sokoto, Oyo, Plateau and Ondo states grouped according to the historical distribution of PPRV together with the Nigerian 75/1 strain of lineage II, while other strains from Sokoto, Oyo, Plateau, Akwa-Ibom, Adamawa, Kaduna, Lagos, Bauchi, Niger and Kano states grouped together with the East African and Asian strains of lineage IV. This finding confirms that both lineage II and IV strains of PPRV are circulating in Nigeria. Previously, only strains of lineage II were found to be present in the country.