The effects of long-term hexabromocyclododecanes contamination on microbial communities in the microcosms
The effects of long-term hexabromocyclododecanes contamination on microbial communities in the microcosms
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DOI：
10.1016/j.chemosphere.2023.138412
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发表时间：
2023-03-20
期刊：
影响因子：
8.8
通讯作者：
Hu，Zhong
中科院分区：
文献类型：
--
作者：
Yu，Fei;Luo，Wenqi;Hu，Zhong
微生物群落对六溴环十二烷（hbcd）长期污染的适应性尚未得到很好的研究。我们之前的研究发现，在8个月的时间里，两种不同红树林沉积物的微观环境中hbcd的污染导致了严重的酸化（pH2-3）。本研究重新分析了之前的测序数据，并将其与20个月后的数据进行了比较，以研究微生物群落在hbcd和酸化胁迫下的适应特性。它假设重组是基于分类群的适应度。结果表明，真核生物和真菌可能对这些退化的生境具有更好的适应能力。真核taxaeufallia和syncystis，真菌taxaickerhamomys在污染20个月后才检出。真核taxacaloneia和nitzschia以及真菌taxatalaromyia在大多数微生物群落中占主导地位（14.467 ~ 95.941%）。功能组成依赖于沉积物，比群落重组更具差异性。网络分析和共现分析表明，在长期胁迫下，嗜酸异构体和嗜酸菌之间的关系正相关。参与抗hbcd酸化和毒性的嗜酸分类群和基因得到了丰富，如细菌acidisomaand acidiphilium、古单胞菌和真核细菌nitzschia，以及基因kdpc、odc1、polA、gst和sod-2。这些基因参与氧化应激反应、能量代谢、DNA损伤修复、钾转运和脱羧。这表明微生物群落可能通过多种途径来应对来自hbcd和酸化的压力。本研究揭示了hbcd污染和酸化长期胁迫下微生物群落的进化。
The adaptation of microbial community to the long-term contamination of hexabromocyclododecanes (HBCDs) has not been well studied. Our previous study found that the HBCDs contamination in the microcosms constructed of sediments from two different mangrove forests in 8 months resulted in serious acidification (pH2-3). This study reanalyzed previous sequencing data and compared them with data after 20 months to investigate the adaptive properties of microbial communities in the stress of HBCDs and acidification. It hypothesized that the reassembly was based on the fitness of taxa. The results indicated that eukaryotes and fungi might have better adaptive capacity to these deteriorated habitats. Eukaryotic taxaEufalliaandSyncystis, and fungal taxaWickerhamomyceswere only detected after 20 months of contamination. Moreover, eukaryotic taxaCaloneisandNitzschia, and fungal taxaTalaromyceswere dominant in most of microbial communities (14.467–95.941%). The functional compositions were sediment-dependent and more divergent than community reassemblies. Network and co-occurrence analysis suggested that acidophiles such asAcidisomaandAcidiphiliumwere gaining more positive relations in the long-term stress. The acidophilic taxa and genes involved in resistance to the acidification and toxicity of HBCDs were enriched, for example, bacteriaAcidisomaandAcidiphilium, archaeaThermogymnomonas, and eukaryotesNitzschia, and geneskdpC,odc1, polA,gst, andsod-2. These genes involved in oxidative stress response, energy metabolism, DNA damage repair, potassium transportation, and decarboxylation. It suggested that the microbial communities might cope with the stress from HBCDs and acidification via multiple pathways. The present research shed light on the evolution of microbial communities under the long-term stress of HBCDs contamination and acidification.