Physiological response to isoproterenol and coupling of beta-adrenergic receptors in young and elderly human subjects.
Physiological response to isoproterenol and coupling of beta-adrenergic receptors in young and elderly human subjects.
复制标题
年轻和老年受试者对异丙肾上腺素的生理反应和β-肾上腺素能受体的偶联。
DOI：
10.1093/geronj/44.3.m100
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Davies,AO
中科院分区：
文献类型：
--
作者：
Montamat,SC;Davies,AO
老年人肾上腺素能反应性降低。本研究的目的是评估人中性粒细胞制剂中β -肾上腺素能受体的偶联，并探讨该生化参数与年轻人和老年人异丙肾上腺素给药后心率反应的关系。15名年轻人（23-39岁）和11名老年人（59-82岁）参与了这项研究。计算了低亲和状态和高亲和状态受体的解离常数之比KL/ khs，以确定β -肾上腺素能受体系统中的偶联。KL/KHand年龄呈负相关。测定I25HR，即使心率每分钟提高25次所需的异丙肾上腺素量；该参数随年龄增长而增加。多元回归分析显示，I25HR与年龄的关系最为密切，但随着年龄的变化，β -肾上腺素能受体对激动剂的亲和力和KL/KH均下降。
Diminished adrenergic responsiveness occurs in aged humans. The purpose of this study was to evaluate the coupling of beta-adrenergic receptors from human neutrophil preparations, and to investigate the relationship of this biochemical parameter to heart rate response after isoproterenol administration in young and elderly individuals. Fifteen young (aged 23–39 years) and eleven elderly (aged 59–82 years) volunteers participated in the study. The ratio, KL/KHrepresenting the ratio of dissociation constants for the low and high affinity state receptors, was calculated to determine coupling in the beta-adrenergic receptor system. An inverse correlation was observed between KL/KHand age. I25HR, representing the amount of isoproterenol required to elevate heart rate by 25 beats per minute, was determined; this parameter increased with age. Multiple regression analysis revealed that I25HR was most dependent upon age, but the changes with age were accompanied by diminution of both beta-adrenergic receptor affinity for agonist and in KL/KH.