Immunologic characterization of hairy cell leukemias in continuous culture.
Immunologic characterization of hairy cell leukemias in continuous culture.
复制标题
连续培养中毛细胞白血病的免疫学特征。
DOI：
10.4049/jimmunol.120.3.777
复制
发表时间：
1978
影响因子：
4.4
通讯作者：
David W. Golde
中科院分区：
文献类型：
--
作者：
Andrew J. Saxon;Ronald H. Stevens;Shirley G. Quan;David W. Golde
本文研究了毛细胞白血病细胞系连续建立的三个细胞系的免疫生物学特性。所有三个细胞系继续产生抗酒石酸酸性磷酸酶，毛细胞的酶标记。这些细胞系中有两种是B淋巴细胞。它们携带Fc和C受体，具有表面和内部免疫球蛋白，并且不形成自发的绵羊红细胞红细胞。采用生物合成放射性标记免疫球蛋白的实验证明了这两种毛细胞系中每一种的独特免疫球蛋白动力学。其中一个细胞系与其来源的原始毛细胞非常相似，而另一个B淋巴毛细胞系在产生的免疫球蛋白同种型中经历了转换。第三个毛细胞白血病细胞系是胸腺衍生的。这些细胞形成自发的绵羊红细胞，不携带Fc或C受体。自发性绵羊红细胞花环形成细胞含有抗酒石酸酸性磷酸酶。它们在体外不合成免疫球蛋白。毛细胞白血病细胞在永久细胞培养中保持其免疫生物学特性，并为深入研究这些不寻常的细胞提供了机会。
The immunobiologic characteristics of three continuous cell lines established from hairy cell leukemia cells were investigated. All three cell lines continued to produce tartrate-resistant acid phosphatase, the enzymatic marker of hairy cells. Two of these cell lines were B lymphoid in nature. They carried Fc and C receptors, had surface and internal immunoglobulin, and did not form spontaneous sheep red blood cell rosettes. Experiments employing biosynthetic radiolabeling of immunoglobulin demonstrated distinctive immunoglobulin kinetics for each of these two hairy cell lines. One cell line remained quite similar to the original hairy cells from which it was derived whereas the other B lymphoid hairy cell line had undergone a switch in the immunoglobulin isotype produced. The third hairy cell leukemia line was shown to be of thymic derivation. These cells formed spontaneous sheep red blood cell rosettes and did not carry Fc or C receptors. The spontaneous sheep red blood cell rosette-forming cells contained tartrate-resistant acid phosphatase. They did not possess surface on internal immunoglobulin and did not synthesize immunoglobulin in vitro. Hairy cell leukemia cells maintained in permanent cell culture retain their immunobiologic properties and offer the opportunity for indepth study of these unusual cells.