Analysis of the organophosphate-induced electromyographic response to repetitive nerve stimulation: paradoxical response to edrophonium and D-tubocurarine.
Analysis of the organophosphate-induced electromyographic response to repetitive nerve stimulation: paradoxical response to edrophonium and D-tubocurarine.
复制标题
有机磷引起的对重复神经刺激的肌电反应分析：对依酚铵和 D-筒箭毒碱的矛盾反应。
DOI：
10.1002/mus.880141207
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发表时间：
1991
期刊：
影响因子：
3.4
通讯作者：
Soliven,BC
中科院分区：
文献类型：
--
作者：
Maselli,RA;Soliven,BC
我们通过对注射二异丙基氟磷酸盐的大鼠进行体内和体外电生理学研究，研究了急性有机磷中毒（OPI）的机制。中毒的大鼠表现出无力、对单一刺激的重复复合肌肉动作电位 (CMAP) 反应，以及对重复神经刺激的反应减弱，这在第二次 CMAP 时最为明显。服用依酚铵后，这种减少更加严重，但被 D-筒箭毒碱完全逆转。体外微电极研究表明微型终板电位（MEPP）的幅度或终板电位（EPP）的量子含量没有减少。然而，MEPPs 和 EPPs 的半衰时间显着延长。刺激序列通过延长 EPP 总和的“阶梯现象”诱导持续的终板去极化，这种现象被 edrophonium 增强，并被 D-筒箭毒碱消除。这些结果表明持续的终板去极化可以直接解释急性 OPI 的减少和减弱。
We studied the mechanism underlying acute organophosphate intoxication (OPI) through in‐vivo and in‐vitro electrophysiologic studies in rats injected with diisopropylfluorophosphate. Intoxicated rats showed weakness, repetitive compound muscle action potentials (CMAPs) in response to a single stimulus, and decremental response to repetitive nerve stimulation that was most pronounced at the second CMAP. The decrement was worsened with the administration of edrophonium, and was completely reversed by D‐tubocurarine. In‐vitro microelectrode studies showed no reduction in the amplitude of miniature endplate potentials (MEPPs) or in the quantal content of end‐plate potentials (EPPs). However, the half‐decay times of MEPPs and EPPs were significantly prolonged. Trains of stimuli induced sustained endplate depolarization via a “staircase phenomenon” of summation of prolonged EPPs, which was enhanced by edrophonium and abolished by D‐tubocurarine. These results indicate that sustained end‐plate depolarization can directly account for the decrement and weakness in acute OPI.