Characterizing non-photochemical quenching in leaves through fluorescence lifetime snapshots
Characterizing non-photochemical quenching in leaves through fluorescence lifetime snapshots
复制标题
通过荧光寿命快照表征叶子中的非光化学猝灭
DOI：
10.1007/s11120-015-0104-2
复制
发表时间：
2015
影响因子：
3.7
通讯作者：
G. Fleming
中科院分区：
文献类型：
--
作者：
E. Sylak;J. Zaks;K. Amarnath;M. Leuenberger;G. Fleming
我们描述了一种技术来测量完整的叶片在适应强光和随后的放松黑暗条件下的荧光衰减曲线。我们展示了如何确保光系统II反应中心关闭，并比较野生型拟南芥与传统的脉冲幅度调制（PAM）荧光测量数据。与PAM测量不同，寿命测量对光漂白或叶绿体屏蔽不敏感，并且荧光衰减的形式提供了额外的信息来测试完整叶片中激发动力学的定量模型。
We describe a technique to measure the fluorescence decay profiles of intact leaves during adaptation to high light and subsequent relaxation to dark conditions. We show how to ensure that photosystem II reaction centers are closed and compare data for wild type Arabidopsis thaliana with conventional pulse-amplitude modulated (PAM) fluorescence measurements. Unlike PAM measurements, the lifetime measurements are not sensitive to photobleaching or chloroplast shielding, and the form of the fluorescence decay provides additional information to test quantitative models of excitation dynamics in intact leaves.