A new method using acid to clean and a technique for preparation of eggs of biting midges (Diptera: Ceratopogonidae) for Scanning Electron Microscope
A new method using acid to clean and a technique for preparation of eggs of biting midges (Diptera: Ceratopogonidae) for Scanning Electron Microscope
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DOI：
10.3157/0002-8320-134.3.471
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发表时间：
2008-09
期刊：
影响因子：
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通讯作者：
M. Ronderos;F. Díaz;P. Sarmiento
中科院分区：
文献类型：
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作者：
M. Ronderos;F. Díaz;P. Sarmiento
摘要描述了一种新的卵清理方法，该方法是一种新的卵清理方法。在新的程序中，不同状态的卵子，具有不同的绒毛膜超微结构，在10%乙醇酸(CH2OH-COOH)和20%扁桃酸(C6H5.CHOH.COOH)中浸泡1-4分钟。用两种酸制备的鸡蛋，然后用蒸馏水中和，放入饱和的苯酚溶液中，然后通过一组酒精溶液。最后进行临界点处理，实现了金的金属化。在准备好后，这组卵子在真空中保持其自然形态的时间足够长，以获得绒毛膜外层的良好显微照片。
Abstract A new procedure for clearing eggs belonging to genera of Ceratopogonidae is described. In the new procedure eggs in different states, with different ultra structures of the exochorion, were immersed in 10% glycolic acid (CH2OH-COOH) and 20 % mandellic acid (C6H5.CHOH.COOH) for 1–4 minutes. Eggs, prepared by both acids and subsequently neutralized by distilled water, were placed in a saturated solution of phenol, and then passed through a battery of alcohol solutions. Finally, critical point was performed and gold metallized. After preparation the eggs of this group retained their natural form under vacuum long enough to yield good microphotographs of the outer layer of the chorion.