Production of hydroxyl radicals from Fe-containing fine particles in Guangzhou, China
Production of hydroxyl radicals from Fe-containing fine particles in Guangzhou, China
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中国广州利用含铁细颗粒生产羟基自由基
DOI：
10.1016/j.atmosenv.2015.10.057
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发表时间：
2015-12-01
影响因子：
5
通讯作者：
Xu, Zhencheng
中科院分区：
文献类型：
--
作者：
Ma, Shexia;Ren, Ke;Xu, Zhencheng
环境细颗粒物产生的活性氧（ROS）与PM2.5中可溶性过渡金属含量相关，这也与颗粒物介导的心肺毒性有明显的关联。羟基自由基（（OH）-O-中心点）是通过氧化还原活性颗粒组分的化学反应产生的最有害的ROS种类。大气Fe作为大气中过渡金属的主要存在形式，与大气颗粒物中（OH）-O中心点的产生有关。结果表明，广州市夏、冬两季大气颗粒物中含铁颗粒物（18,730个）平均约占3.7%，可分为富铁颗粒物、富金属颗粒物、富钠钾颗粒物和富尘颗粒物4类。富铁类占61%，其次为富尘类（14%）、富金属类（13%）。高钾（12%）。铁氧化物富集在富铁类中。在替代肺液（SLF）中定量测定了广州（GZ）收集的含铁细颗粒物诱导的（OH）-O-中心点的生成，发现在存在四种抗氧化剂的情况下，含铁细颗粒物通常更容易生成（OH）-O-中心点（200 μ M抗坏血酸盐、300 μ M柠檬酸盐、100 μ M还原型L-谷胱甘肽和100 μ M尿酸）。我们的样本中产生的年平均（OH）-O-中心点量为132.98 +/- 27.43 nmol（OH）-O-中心点mg（-1）PM2.5。（OH）-O-中心点的产生具有明显的季节性，夏季含量较高，冬季含量较低。通过ICP-MS对广州市PM2. 5中总金属和超低频可溶性金属含量的测定，发现广州市PM2. 5中活性氧活性与可电离铁通过芬顿型反应有关。人肺衬里液中PM2.5来源的（OH）-O-中心点的预期负荷表明，广州典型的每日颗粒物暴露已经是一个问题，它可能产生更高水平的（OH）-O-中心点，导致更高的细胞毒性。(C)2015爱思唯尔有限公司版权所有。
Reactive oxygen species (ROS) production from ambient fine particles has been correlated with the soluble transition metal content of PM2.5, which also has clear association with particle-mediated cardiopulmonary toxicity. Hydroxyl radical ((OH)-O-center dot) is the most harmful ROS species through chemical reactions of redox-active particle components. Atmospheric Fe, as the dominant species of the transition metals in the atmosphere, is associated with (OH)-O-center dot generation in ambient particle extracts. Our results revealed that Fe-containing particles (18,730 in total number) contributed approximately 3.7% on average to all detected particles throughout the summer and winter sampling period in Guangzhou, which was clustered into four distinct particle classes, including Fe-rich, Metal-rich, NaK-rich and Dust-rich. Fe-rich class was the dominant one with a fraction of 61%, followed by Dust-rich (14%), Metal-rich (13%). and NaK-rich (12%). The iron oxide was enriched in the Fe-rich class. (OH)-O-center dot generation induced by Fe-containing fine particles collected in Guangzhou (GZ) was quantified in a surrogate lung fluid (SLF), and it was found that Fe-containing fine particles were generally much reactive in generating (OH)-O-center dot in the presence of four antioxidants (200 mu M ascorbate, 300 mu M citrate, 100 mu M reduced L-glutathione, and 100 mu M uric acid). The annual average (OH)-O-center dot amount produced in our samples was 132.98 +/- 27.43 nmol (OH)-O-center dot mg(-1) PM2.5. (OH)-O-center dot production had a clear seasonal pattern with higher amount in summer and lower in winter. By measuring the amount of total and SLF-soluble metal in our PM2.5 samples using ICP-MS, we found that ROS activities were associated with the ionizable Fe through Fenton type reactions in the Guangzhou PM2.5. Expected burdens of PM2.5 derived (OH)-O-center dot in human lung lining fluid suggests that typical daily particulate matter exposure in Guangzhou is already a concern, and it could produce much higher levels of (OH)-O-center dot, leading to higher cytotoxicity. (C) 2015 Elsevier Ltd. All rights reserved.