HIGH-EFFICIENCY POLYOMA DNA TRANSFECTION OF CHLOROQUINE TREATED-CELLS
HIGH-EFFICIENCY POLYOMA DNA TRANSFECTION OF CHLOROQUINE TREATED-CELLS
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DOI：
10.1093/nar/11.5.1295
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发表时间：
1983-01-01
影响因子：
14.9
通讯作者：
MAGNUSSON, G
中科院分区：
文献类型：
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作者：
LUTHMAN, H;MAGNUSSON, G
在多瘤 DNA 转染的最初几个小时内，对啮齿动物细胞进行氯喹处理可增加表达病毒功能的细胞比例。使用 DEAE-葡聚糖和磷酸钙共沉淀方法吸收 DNA 后观察到了这种效果。暴露于氯喹使转染小鼠细胞的比例增加至约 40'。从一百万个这样的细胞的培养物中，可以分离出微克量的新合成的病毒DNA。类似地，氯喹处理后，多瘤DNA转染后的大鼠细胞的转化频率大约增加了6倍。该化合物的作用在线性多瘤 DNA 转染中更为明显，表明氯喹可抑制细胞吸收的 DNA 降解。
Chloroquine treatment of rodent cells during the first hours of polyoma DNA transfection increase the fraction of cells expressing viral functions. The effect has been observed after DNA absorption using both the DEAE-dextran and calcium phosphate co-precipitation methods. Exposure to chloroquine increased the proportion of transfected mouse cells to approximately 40′. From a culture of one million such cells, microgram quantities of newly synthesized viral DNA could be isolated. Similarly, the transformation frequency of rat cells following polyoma DNA transfection was approximately 6-fold increased by chloroquine treatment. The effect of the compound was even more pronounced in transfections with linear forms of polyoma DNA, suggesting that chloroquine inhibits degradation of DNA absorbed by the cells.