A bacterial isolate from the Black Sea oxidizes sulfide with manganese(IV) oxide
A bacterial isolate from the Black Sea oxidizes sulfide with manganese(IV) oxide
复制标题
DOI：
10.1073/pnas.1906000116
复制
发表时间：
2019-06-18
影响因子：
11.1
通讯作者：
Schulz-Vogt, Heide N.
中科院分区：
文献类型：
--
作者：
Henkel, Jan V.;Dellwig, Olaf;Schulz-Vogt, Heide N.
锰是地球上最丰富的氧化还原敏感金属之一。已知一些微生物使用四氧化二锰 (MnO2) 作为电子受体来氧化有机化合物或氢气 (H-2)，但迄今为止已建议使用硫化物 (H2S)，但尚未得到证实。在这里，我们报道了一种细菌分离物，它可以自养生长，并将 MnO2 的还原与 H2S 或硫代硫酸盐 (S2O32-) 的氧化结合起来以产生能量。该分离株源自黑海，是硫单胞菌属的一个物种，通常在含硫环境附近以大量细胞数量出现[Y. Han, M. Perner，前。微生物。 6、989（2015）]。 H2S和S2O32-完全氧化成硫酸盐(SO42-)，没有中间体积累。在培养物中，Mn(IV) 还原通过 Mn(III) 进行，最后沉淀出富含 Ca 的碳酸 Mn(II) [Mn(Ca)CO3]。与使用有机电子供体或 H-2 的 Mn 还原细菌相反，没有观察到 Fe 氧化物支持生长，这可能表明基因组不完整或细胞外电子转移的途径不同。
Mn is one of the most abundant redox-sensitive metals on earth. Some microorganisms are known to use Mn(IV) oxide (MnO2) as electron acceptor for the oxidation of organic compounds or hydrogen (H-2), but so far the use of sulfide (H2S) has been suggested but not proven. Here we report on a bacterial isolate which grows autotrophically and couples the reduction of MnO2 to the oxidation of H2S or thiosulfate (S2O32-) for energy generation. The isolate, originating from the Black Sea, is a species within the genus Sulfurimonas, which typically occurs with high cell numbers in the vicinity of sulfidic environments [Y. Han, M. Perner, Front. Microbiol. 6, 989 (2015)]. H2S and S2O32- are oxidized completely to sulfate (SO42-) without the accumulation of intermediates. In the culture, Mn(IV) reduction proceeds via Mn(III) and finally precipitation of Ca-rich Mn(II) carbonate [Mn(Ca)CO3]. In contrast to Mn-reducing bacteria, which use organic electron donors or H-2, Fe oxides are not observed to support growth, which may either indicate an incomplete gene set or a different pathway for extracellular electron transfer.