Engineering growth factors for regenerative medicine applications.
Engineering growth factors for regenerative medicine applications.
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DOI：
10.1016/j.actbio.2015.11.007
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发表时间：
2016-01
影响因子：
9.7
通讯作者：
Cochran JR
中科院分区：
文献类型：
--
作者：
Mitchell AC;Briquez PS;Hubbell JA;Cochran JR
生长因子是重要的形态发生蛋白，可指导细胞行为并指导组织修复和更新。尽管它们的治疗潜力在再生医学应用中具有巨大的前景，但将生长因子转化为临床治疗却受到一些局限性的阻碍，包括蛋白质稳定性差、重组表达产量低和疗效欠佳。本综述重点介绍了当前为再生医学应用设计集成且有效的基于生长因子的疗法的工具、技术和方法。第一部分描述了合理的组合蛋白质工程方法，这些方法已用于改善生长因子稳定性、表达产量、生物分布和血清半衰期，或改变其细胞运输行为或受体结合亲和力。第二部分重点介绍基于生物材料的优雅系统，其灵感来自于天然细胞外基质环境，该系统是为了将生长因子有效地在空间和时间上传递到细胞表面受体而开发的。尽管看起来截然不同，但这两种方法具有高度互补性，并且涉及在开发临床应用的最佳材料时并行考虑的分子设计和工程原理。
Growth factors are important morphogenetic proteins that instruct cell behavior and guide tissue repair and renewal. Although their therapeutic potential holds great promise in regenerative medicine applications, translation of growth factors into clinical treatments has been hindered by limitations including poor protein stability, low recombinant expression yield, and suboptimal efficacy. This review highlights current tools, technologies, and approaches to design integrated and effective growth factor-based therapies for regenerative medicine applications. The first section describes rational and combinatorial protein engineering approaches that have been utilized to improve growth factor stability, expression yield, biodistribution, and serum half-life, or alter their cell trafficking behavior or receptor binding affinity. The second section highlights elegant biomaterial-based systems, inspired by the natural extracellular matrix milieu, that have been developed for effective spatial and temporal delivery of growth factors to cell surface receptors. Although appearing distinct, these two approaches are highly complementary and involve principles of molecular design and engineering to be considered in parallel when developing optimal materials for clinical applications.
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影响因子：
3.3
作者：
Ferrara N
通讯作者：
Ferrara N
影响因子：
14
作者：
Ehrbar, Martin;Rizzi, Simone C.;Lutolf, Matthias P.
通讯作者：
Lutolf, Matthias P.
DOI：
10.1091/mbc.2.8.599
发表时间：
1991-08-01
期刊：
CELL REGULATION
影响因子：
--
作者：
EBNER, R;DERYNCK, R
通讯作者：
DERYNCK, R
影响因子：
4.8
作者：
FRENCH, AR;TADAKI, DK;LAUFFENBURGER, DA
通讯作者：
LAUFFENBURGER, DA
影响因子：
46.9
作者：
Coco, WM;Encell, LP;Monticello, DJ
通讯作者：
Monticello, DJ