Evaluation of sunscreen products using a reconstructed skin model exposed to simulated daily ultraviolet radiation: relevance of filtration profile and SPF value for daily photoprotection
Evaluation of sunscreen products using a reconstructed skin model exposed to simulated daily ultraviolet radiation: relevance of filtration profile and SPF value for daily photoprotection
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DOI：
10.1111/j.1600-0781.2008.00370.x
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发表时间：
2008-10-01
影响因子：
2.6
通讯作者：
Bernerd, Francois
中科院分区：
文献类型：
--
作者：
Lejeune, Francois;Christiaens, Francois;Bernerd, Francois
背景：最近定义的标准日紫外线辐射（DUVR），使我们能够重现非天顶太阳照射条件。暴露于模拟DUVR诱导人体皮肤的生物损伤，建议需要一个适当的photoprotectory.Methods：防晒产品进行了评估，使用人类重建的皮肤在体外。在暴露于增加剂量的DUVR的皮肤模型上测试具有相似的防晒（烧伤）保护因子（SPF）值（类似于15）但在UVA范围内具有不同的透射曲线的两种商业防晒剂（A和B）。另一对防晒霜也进行了测试。一种（产品C）具有类似于18的SPF，具有良好平衡的UVB-UVA分布，另一种（产品D）具有类似于27的SPF，具有低UVA吸收。通过（i）组织学、（ii）真皮成纤维细胞的波形蛋白免疫染色和（iii）基质金属蛋白酶（MMP）-1分泌的分析来评估生物学参数。结果：与产品B和D相比，产品A和C分别在成纤维细胞改变和MMP-1释放方面对DUVR具有更好的保护作用。为了确保有效的日常光保护免受DUVR，产品的过滤特性应与足够的UVA吸收水平保持良好平衡。关于本研究中评估的终点，我们的数据表明，较高的SPF值并不能补偿低UVA过滤。
Background: The recent definition of a standard daily ultraviolet radiation (DUVR) has allowed us to reproduce non-zenithal sun exposure conditions. Exposure to simulated DUVR induces biological damage in human skin, suggesting the need for an appropriate photoprotection.Methods: Sunscreen products were evaluated using human reconstructed skin in vitro. Two commercial sunscreens (A and B) having similar sun (burn) protection factor (SPF) values (similar to 15) but different profiles of transmission over the UVA range were tested on skin models exposed to increasing doses of DUVR. Another pair of sunscreens was also tested. One (product C) had an SPF similar to 18 with a well-balanced UVB-UVA profile and the other (product D) an SPF of similar to 27 with low UVA absorption. Biological parameters were assessed by (i) histology, (ii) vimentin immunostaining for dermal fibroblasts, and (iii) analysis of matrix metalloprotease (MMP)-1 secretion.Results: Products A and C gave better protection from DUVR with regard to fibroblast alterations and MMP-1 release compared with products B and D, respectively.Conclusion: To ensure an efficient daily photoprotection from DUVR, the filtration profile of the product should be well balanced with a sufficient level of UVA absorption. With regard to end points evaluated in this study, our data suggest that a higher SPF value does not compensate for low UVA filtration.