Human post-implantation embryo collection: medical and surgical techniques
Human post-implantation embryo collection: medical and surgical techniques
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DOI：
10.1016/s0378-3782(97)00118-7
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发表时间：
1998-07-10
影响因子：
2.5
通讯作者：
Strachan, T
中科院分区：
文献类型：
--
作者：
Bullen, PJ;Robson, SC;Strachan, T
关于人类早期发育的遗传控制的知识是有限的，大多数信息都是从动物模型的研究中推断出来的。有令人信服的证据表明，在人类胚胎中进行基因表达研究有望大大提高我们对胚胎形成和畸形的理解。这些研究需要系统和协调地收集、储存和研究人类胚胎。我们已经成功地从正在进行终止妊娠（TOP）的病例中收集了完整的胚胎。胚胎材料收集从62%的尝试，使用超声引导下进行手术抽吸技术。医学终止妊娠后的采集率较低（41%），尽管两种方法未破裂胚胎的比例相同（26%）。手术抽吸提供了Carnegie阶段（CS）16-22之间的完整胚胎，而早期发育阶段则从医疗终止中收集。我们收集了60多个完整的标本，跨越卡内基阶段10至22（约21至53天的发展），涵盖了关键发展事件的巨大窗口，因此代表了一个令人兴奋和宝贵的研究资源。(C)1998爱思唯尔科学爱尔兰有限公司保留所有权利。
Knowledge of the genetic control of early human development is limited with most information being extrapolated from studies in animal models. There is compelling evidence that undertaking gene expression studies in human embryos can be expected to dramatically enhance our understanding of embryonic formation and malformation. Such studies require the systematic and coordinated collection, storage and study of human embryos. We have successfully collected intact embryos from cases undergoing termination of pregnancy (TOP). Embryonic material was collected from 62% of attempts using a technique of surgical aspiration carried out under ultrasound guidance. Collection rates were lower after medical termination of pregnancy (41%) although the proportion of undisrupted embryos was identical with the two methods (26%). Surgical aspiration provided intact embryos between Carnegie stages (CS) 16-22 while earlier developmental stages were collected from medical terminations. Our collection of over 60 intact specimens, spanning Carnegie stages 10 to 22 (about 21 to 53 days of development) covers a huge window of critical developmental events and hence represents an exciting and valuable research resource. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.