Vitamin D deficiency in gestational diabetes mellitus and the role of the placenta
Vitamin D deficiency in gestational diabetes mellitus and the role of the placenta
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DOI：
10.1016/j.ajog.2013.08.015
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发表时间：
2013-12-01
影响因子：
9.8
通讯作者：
Kim, Hai-Joong
中科院分区：
文献类型：
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作者：
Cho, Geum Joon;Hong, Soon-Cheol;Kim, Hai-Joong
目的：本研究旨在评估母体维生素D水平与妊娠期糖尿病（GDM）之间的关系，以及胎盘维生素D受体（VDR）、CYP24A和CYP27B1产生的差异。
研究设计：本研究纳入40名正常孕妇和20名妊娠期糖尿病孕妇。采用酶联免疫吸附测定法测量血清25 - 羟基维生素D（25[OH]D）水平。分别采用实时聚合酶链反应和蛋白质印迹法评估胎盘中VDR、CYP27B1和CYP24A1的表达及产生情况。
结果：我们发现27.5%的正常孕妇和85%的妊娠期糖尿病孕妇存在维生素D缺乏，其血清25(OH)D水平……
OBJECTIVE: The aim of this study was to evaluate the relationships between maternal vitamin D levels and gestational diabetes mellitus (GDM) and differences in the placental production of vitamin D receptor (VDR), CYP24A, and CYP27B1.STUDY DESIGN: Forty normal pregnant women and 20 women with GDM were included in this study. Serum levels of 25-hydroxyvitamin D (25[OH]D) were measured with enzyme-linked immunosorbent assay. The expression and production of VDR, CYP27B1, and CYP24A1 in the placenta were evaluated with real time-polymerase chain reaction and Western blot, respectively.RESULTS: We found that 27.5% of normal pregnant women and 85% of women with GDM had vitamin D deficiency, with serum 25(OH)D levels