Symbiont population control by host-symbiont metabolic interaction in Symbiodiniaceae-cnidarian associations
Symbiont population control by host-symbiont metabolic interaction in Symbiodiniaceae-cnidarian associations
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DOI：
10.1038/s41467-019-13963-z
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发表时间：
2020-01
影响因子：
16.6
通讯作者：
T. Xiang;E. Lehnert;Robert E. Jinkerson;S. Clowez;Rick G. Kim;Jan C. DeNofrio;J. Pringle;A. Grossman-A.-Gr
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文献类型：
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作者：
T. Xiang;E. Lehnert;Robert E. Jinkerson;S. Clowez;Rick G. Kim;Jan C. DeNofrio;J. Pringle;A. Grossman-A.-Gr
在刺胞动物-共生藻科共生体中，宿主内的藻类内共生体的数量控制是维持共生关系的必要条件。然而，这种种群控制的分子机制尚不清楚。在这里，我们表明，刺胞宿主使用氮限制作为一个主要的机制来控制内共生体的人口。氮的获取和同化成绩单成为共生Breviolum minutumalgae升高，因为他们达到高密度内的海葵主机Exaiptasia苍白。这些相同的转录本增加了缺乏氮的自由生活的藻类。共生藻类也有一个升高的碳-氮比和转向代谢清除氮从嘌呤相对于自由生活algae.Exaiptasiaglutamine合成酶和谷氨酸合酶的成绩单伴随着增加的藻类内共生体人口，这表明增加的能力，主机同化铵。这些结果表明，藻类的生长和复制在磷石膏控制的访问氮，这成为限制的藻类，因为它们的人口在主机内增加。
In cnidarian-Symbiodiniaceae symbioses, algal endosymbiont population control within the host is needed to sustain a symbiotic relationship. However, the molecular mechanisms that underlie such population control are unclear. Here we show that a cnidarian host uses nitrogen limitation as a primary mechanism to control endosymbiont populations. Nitrogen acquisition and assimilation transcripts become elevated in symbioticBreviolum minutumalgae as they reach high-densities within the sea anemone hostExaiptasia pallida. These same transcripts increase in free-living algae deprived of nitrogen. Symbiotic algae also have an elevated carbon-to-nitrogen ratio and shift metabolism towards scavenging nitrogen from purines relative to free-living algae.Exaiptasiaglutamine synthetase and glutamate synthase transcripts concomitantly increase with the algal endosymbiont population, suggesting an increased ability of the host to assimilate ammonium. These results suggest algal growth and replication in hospite is controlled by access to nitrogen, which becomes limiting for the algae as their population within the host increases.