Amorphous silica nanoparticles enhance cross-presentation in murine dendritic cells
Amorphous silica nanoparticles enhance cross-presentation in murine dendritic cells
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DOI：
10.1016/j.bbrc.2012.09.095
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发表时间：
2012-10-26
影响因子：
3.1
通讯作者：
Tsutsumi, Yasuo
中科院分区：
文献类型：
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作者：
Hirai, Toshiro;Yoshioka, Yasuo;Tsutsumi, Yasuo
纳米材料具有独特的物理化学性质和新颖的功能，在各个领域的应用日益广泛。确保核材料的安全是一项紧迫的任务。最近，我们报道了无定形二氧化硅纳米颗粒（nSPs），最广泛使用的纳米材料之一，增强抗原特异性细胞免疫反应，因此可能会加重免疫疾病。因此，为了确保设计更安全的nSP，现在需要研究nSP对树突状细胞中抗原呈递的影响，树突状细胞是适应性免疫应答的中央指挥官。在这里，我们发现直径为70和100 nm的nSP增强了外源性抗原从内体进入胞质溶胶并诱导交叉呈递，而亚微米尺寸的二氧化硅颗粒（>100 nm）则没有。此外，我们表明，表面修饰的nSP抑制交叉介绍。虽然需要进一步的研究来调查表面修饰的nSP是否抑制体内免疫调节作用，但目前的结果表明，适当调节nSP的特性，如大小和表面性质，对于设计更安全的nSP至关重要。(C)2012 Elsevier Inc. All rights reserved.
Nanomaterials (NMs) exhibit unique physicochemical properties and innovative functions, and they are increasingly being used in a wide variety of fields. Ensuring the safety of NMs is now an urgent task. Recently, we reported that amorphous silica nanoparticles (nSPs), one of the most widely used NMs, enhance antigen-specific cellular immune responses and may therefore aggravate immune diseases. Thus, to ensure the design of safer nSPs, investigations into the effect of nSPs on antigen presentation in dendritic cells, which are central orchestrators of the adaptive immune response, are now needed. Here, we show that nSPs with diameters of 70 and 100 nm enhanced exogenous antigen entry into the cytosol from endosomes and induced cross-presentation, whereas submicron-sized silica particles (>100 nm) did not. Furthermore, we show that surface modification of nSPs suppressed cross-presentation. Although further studies are required to investigate whether surface-modified nSPs suppress immune-modulating effects in vivo, the current results indicate that appropriate regulation of the characteristics of nSPs, such as size and surface properties, will be critical for the design of safer nSPs. (C) 2012 Elsevier Inc. All rights reserved.