Vaginal mucosa serves as an inductive site for tolerance
Vaginal mucosa serves as an inductive site for tolerance
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DOI：
10.4049/jimmunol.165.9.5077
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发表时间：
2000-11-01
影响因子：
4.4
通讯作者：
Edwards, RP
中科院分区：
文献类型：
--
作者：
Black, CA;Rohan, LC;Edwards, RP
这些数据表明阴道Ag暴露可诱导耐受性。在这些实验中，给予小鼠含有5mg OVA或盐水的阴道琼脂糖凝胶栓剂6小时。在动情周期的动情期（雌激素主导）或间情期（孕酮主导）阶段给予小鼠栓剂。在接种期间将小鼠束缚以防止Ag的口腔阴道传播。CFA中的OVA。3周后，通过测量足垫肿胀和测量淋巴细胞对Ag的体外增殖来测试小鼠的迟发型超敏反应。使用ELISA，还测量血清Ab反应的幅度。在一些小鼠中，使用与OVA缀合的FITC来追踪蛋白质向全身组织中的传播。与接受生理盐水栓剂的小鼠相比，发情期接受含OVA栓剂的小鼠足垫肿胀程度显著降低。同时在淋巴结淋巴细胞和脾细胞中也观察到Ag特异性增殖反应降低。相反，在发情间期接种的小鼠对Ag的反应无论是通过足垫反应还是体外增殖都没有显示出降低，发情期接种小鼠的血清Ab滴度没有显著降低。这些数据表明，生殖道可以是粘膜诱导耐受的诱导部位。然而，与其他粘膜部位（如肺和胃肠道）不同，生殖道耐受性诱导受尿道调节。
These data demonstrate that tolerance can be induced by vaginal Ag exposure. In these experiments, mice were given vaginal agarose gel suppositories containing either 5 mg OVA or saline for 6 h, Mice were given suppositories either during the estrous (estrogen dominant) or diestrous (progesterone dominant) stage of the estrous cycle. Mice were restrained during the inoculation period to prevent orovaginal transmission of the Ag, After 1 wk, mice were immunized s.c. with OVA in CFA. After 3 wk, mice were tested for delayed-type hypersensitivity responses by measuring footpad swelling and measuring in vitro proliferation of lymphocytes to Ag, Using ELISA, the magnitude of the serum Ab response was also measured. In some mice, FITC conjugated to OVA was used to track the dissemination of the protein into the systemic tissues. The magnitude of footpad swelling was significantly reduced in mice receiving OVA-containing suppositories during estrus compared with mice receiving saline suppositories. Concomitant decreases in the Ag-specific proliferative response were also observed in lymph node lymphocytes and splenocytes. Conversely, mice inoculated during diestrus did not show a decreased response to Ag by either footpad response or in vitro proliferation, Serum Ab titers in the estrus-inoculated mice did not decrease significantly. These data demonstrate that the reproductive tract can be an inductive site for mucosally induced tolerance. However, unlike other mucosal sites such as the lung and gastrointestinal tract, reproductive tract tolerance induction is hormonally regulated.