Synthesis of monodispersed molecularly imprinted polymer particles for high-performance liquid chromatographic separation of cholesterol using templating polymerization in porous silica gel bound with cholesterol molecules on its surface.
Synthesis of monodispersed molecularly imprinted polymer particles for high-performance liquid chromatographic separation of cholesterol using templating polymerization in porous silica gel bound with cholesterol molecules on its surface.
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DOI：
10.1016/j.chroma.2010.09.041
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发表时间：
2010-11
期刊：
影响因子：
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通讯作者：
K. Kitahara;I. Yoshihama;T. Hanada;Hiroko Kokuba;S. Arai
中科院分区：
文献类型：
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作者：
K. Kitahara;I. Yoshihama;T. Hanada;Hiroko Kokuba;S. Arai
在表面用胆固醇功能化的模板硅胶颗粒（粒径：5μm；孔径：10nm）存在下，通过苯乙烯和二乙烯基苯的共聚制备了对胆固醇具有选择性的单分散分子印迹聚合物颗粒，然后用NaOH水溶液溶解胆固醇键合的硅胶。分子印迹聚合物（MIP）颗粒的透射和扫描电子显微照片显示出良好的单分散性和多孔结构。将MIP颗粒填充到高效液相色谱柱中，并与不含胆固醇的苯乙烯和二乙烯基苯制备的非印迹聚合物（NIP）颗粒进行比较，使用胆固醇、胆固醇酯和脂肪酸甲酯评价其对胆固醇的识别能力。 MIP 颗粒对胆固醇和胆固醇酯表现出高亲和力（[分子式：见正文]）。
Monodispersed molecularly imprinted polymer particles selective for cholesterol were prepared by the copolymerization of styrene and divinylbenzene in the presence of template silica gel particles (particle size: 5μm; pore size: 10nm) functionalized with cholesterol on the surface, followed by dissolution of the cholesterol-bonded silica gel with a NaOH aqueous solution. Transmission and scanning electron micrographs of the molecularly imprinted polymer (MIP) particles revealed good monodispersity and porous structure. The MIP particles were packed into a high performance liquid chromatographic column, and its recognition ability of cholesterol was evaluated using cholesterol, cholesterol esters and fatty acid methyl esters by comparison with the non-imprinted polymer (NIP) particles prepared from styrene and divinylbenzene without cholesterol. The MIP particles showed a high affinity for cholesterol and cholesterol esters ( [Formula: see text] ).