Comparative genomic analysis for the presence of potential enterococcal virulence factors in the probiotic Enterococcus faecalis strain Symbioflor 1
Comparative genomic analysis for the presence of potential enterococcal virulence factors in the probiotic Enterococcus faecalis strain Symbioflor 1
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DOI：
10.1016/j.ijmm.2007.02.008
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发表时间：
2007-11-01
影响因子：
4.1
通讯作者：
Chakraborty, Trinad
中科院分区：
文献类型：
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作者：
Domann, Eugen;Hain, Torsten;Chakraborty, Trinad
肠球菌是动物和人类肠道天然微生物群的成员，能够引起机会性感染。它们还被食品工业用作发酵剂，也被制药工业用于保健品和益生菌中。这种两面性的状况需要根据致病特性进行仔细评估，以确保用于生产食品和药品的菌株的安全性。我们对益生菌株粪肠球菌Symbioflor I进行了基因组缺口测序，并展示了与先前测序的致病性临床分离株粪肠球菌V583进行比较基因组分析得出的初步结果。两种菌株之间在基因共线性上总体有很强的保守性，详细分析显示益生菌株的染色体缺失了大片基因组区域，这表明存在基因丢失。Symbioflor I菌株缺失的基因包括编码肠球菌溶素、肠球菌表面蛋白、明胶酶（球菌溶素）以及透明质酸酶和肽类抗生素AS - 48的基因。使用针对相应基因的特异性PCR引物证实了这一数据。然而，检测到了其他肠球菌决定因子，如聚集物质、胶原蛋白粘附蛋白、抵抗氧阴离子的能力以及荚膜形成能力。这些特性的存在可能对Symbioflor I菌株有利，因为它们可能使细菌在黏膜表面定植和增殖，从而赋予其益生菌特性。（c）2007爱思唯尔有限公司。保留所有权利。
Enterococci are members of the natural microbiota of animal and human intestinal tracts and are capable of causing opportunistic infections. They are also used as starter cultures in the food industry as well as in health supplements and probiotics by the pharmaceutical industry. This Janus-faced status requires a careful evaluation on the basis of pathogenic traits to ensure the safety of the strain used to produce food and pharmaceuticals. We performed gapped-genome sequencing of a probiotic strain Enterococcus faecalis Symbioflor I and present initial results deriving from comparative genome analysis with that of the previously sequenced pathogenic clinical isolate E faecalis V583. There was strong overall conservation of synteny between both strains and a detailed analysis revealed the absence of large genomic regions from the chromosome of the probiotic strain, indicating gene loss. Genes absent from the Symbioflor I strain included those encoding the enterococcal cytolysin, enterococcal surface protein, and gelatinase (coccolysin) as well as hyaluronidase and the peptide antibiotic AS-48. This data was confirmed using PCR primers specific for the respective genes. However, other enterococcal determinants such as aggregation substance, collagen adhesion protein, the ability to resist oxygen anions as well as capsule formation were detected. The presence of these traits may be advantageous for the strain Symbioflor I since they potentially enable colonization and proliferation of the bacterium on mucosal surfaces thereby conferring on it probiotic traits. (c) 2007 Elsevier GmbH. All rights reserved.