A transcriptome resource for the deep-sea bacterium Shewanella piezotolerans WP3 under cold and high hydrostatic pressure shock stress
A transcriptome resource for the deep-sea bacterium Shewanella piezotolerans WP3 under cold and high hydrostatic pressure shock stress
复制标题
冷和高静水压冲击应力下深海细菌 Shewanella piezotolerans WP3 的转录组资源
DOI：
10.1016/j.margen.2016.09.004
复制
发表时间：
2016-12-01
期刊：
影响因子：
1.9
通讯作者：
Xiao, Xiang
中科院分区：
文献类型：
--
作者：
Jian, Huahua;Li, Shengkang;Xiao, Xiang
低温和高静水压力是影响深海生态系统的两个最显著的环境因子。生活在这些极端环境中的微生物对低温和高压的适应机制值得研究，在这项研究中，通过DNA微阵列分析评估了深海细菌Shewanella piezotolerans WP 3对冷（0 ℃）和HHP（50 MPa）冲击的全球基因表达模式。结果表明，WP3在冷休克30、60和90 min后分别有22、66和106个基因差异表达，其中16个基因被鉴定为共同差异表达基因。HHP休克30 min和120 min后，分别鉴定出5个和10个DEG基因。DEG模式的系统聚类分析表明，WP3可能采用不同的适应策略来科普冷和HHP冲击胁迫。总之，我们的研究为深海细菌对寒冷和HHP胁迫的反应提供了转录组资源。本研究为进一步研究底栖细菌的环境适应机制奠定了基础。(C)2016爱思唯尔B.V.保留所有权利。
Low temperature and high hydrostatic pressure (HHP) are two of the most remarkable environmental factors influencing deep-sea ecosystem. The adaptive mechanisms of microorganisms which live in these extreme environments to low temperature and high pressure warrant investigation, In this study, the global gene expression patterns of the deep-sea bacterium Shewanella piezotolerans WP3 in response to cold (0 degrees C) and HHP (50 MPa) shock were evaluated through DNA microarray analysis. Results revealed that 22, 66, and 106 genes were differentially expressed after WP3 was respectively exposed to cold shock for 30, 60, and 90 min. Of these genes, 16 genes were identified as common differentially expressed genes (DEGs). After 30 min and 120 min of HHP shock, 5 and 10 genes were respectively identified as DEGs. The hierarchical clustering analysis of the DEG pattern indicated that WP3 may employ different adaptive strategies to cope with cold and HHP shock stress. Taken together, our study provided a transcriptome resource for deep-sea bacterial responses to cold and HHP stress. This study also established a basis for further investigations on environmental adaptive mechanisms utilized by benthic bacteria. (C) 2016 Elsevier B.V. All rights reserved.