Synthesis of soluble elastin by aortic medial cells in culture.
Synthesis of soluble elastin by aortic medial cells in culture.
复制标题
培养的主动脉内侧细胞合成可溶性弹性蛋白。
DOI：
10.1016/s0006-291x(74)80461-4
复制
发表时间：
1974
影响因子：
3.1
通讯作者：
W. Carnes
中科院分区：
文献类型：
--
作者：
P. Abraham;D. W. Smith;W. Carnes
将源自新生猪主动脉培养基的细胞与[3H]脯氨酸和[3H]缬氨酸长期培养，产生标记蛋白，该蛋白用来自缺铜猪主动脉的盐溶性弹性蛋白作为载体进行分离和纯化。标记蛋白在冷条件下可溶于强中性盐溶液。它在37°可逆凝聚，并与载体蛋白一起在6M尿素中的聚丙烯酰胺凝胶电泳中迁移。新合成蛋白的缬氨酸、脯氨酸和羟脯氨酸具有较高的放射性，羟脯氨酸：脯氨酸的活性比与载体蛋白中这些氨基酸的摩尔比（1：10.3）基本相同（1：11），表明它们的同一性。
Incubation of a long term culture of cells derived from newborn pig aortic media with [3H]proline and [3H]valine yielded a labeled protein that was isolated and purified with salt-soluble elastin from copper deficient pig aorta as carrier. The labeled protein was soluble in strong neutral salt solution in the cold. It coacervated reversibly at 37° and migrated in polyacrylamide gel electrophoresis in 6M urea with the carrier protein. The valine, proline and hydroxyproline of the newly synthesized protein had high radioactivity and the ratio of activities of hydroxyproline: proline were essentially the same (1:11) as the molar ratios of these amino acids in the carrier protein (1:10.3), indicating their identity.