Programmable full-adder computations in communicating three-dimensional cell cultures
Programmable full-adder computations in communicating three-dimensional cell cultures
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DOI：
10.1038/nmeth.4505
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发表时间：
2018-01-01
期刊：
影响因子：
48
通讯作者：
Fussenegger, Martin
中科院分区：
文献类型：
--
作者：
Auslander, David;Auslander, Simon;Fussenegger, Martin
合成生物学家已经推进了生物诱导型基因开关的设计，并将其组装成输入可编程电路，使工程人类细胞能够进行算术计算，让人想起电子电路。通过设计一个通用的即插即用分子计算平台，我们已经用遗传程序设计了九种不同的细胞群，每种细胞群都编码了一个定义的计算指令。当组装成3D培养物时，这些工程细胞财团执行可编程的多细胞全加器逻辑以响应三种触发化合物。
Synthetic biologists have advanced the design of trigger-inducible gene switches and their assembly into input-programmable circuits that enable engineered human cells to perform arithmetic calculations reminiscent of electronic circuits. By designing a versatile plug-and-play molecular-computation platform, we have engineered nine different cell populations with genetic programs, each of which encodes a defined computational instruction. When assembled into 3D cultures, these engineered cell consortia execute programmable multicellular full-adder logics in response to three trigger compounds.