Effects of exercise training on nitric oxide synthase in the kidney of spontaneously hypertensive rats
Effects of exercise training on nitric oxide synthase in the kidney of spontaneously hypertensive rats
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DOI：
10.1111/1440-1681.12040
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发表时间：
2013-02-01
影响因子：
2.9
通讯作者：
Kohzuki, Masahiro
中科院分区：
文献类型：
--
作者：
Ito, Daisuke;Ito, Osamu;Kohzuki, Masahiro
众所周知，运动训练对人类和患有高血压的动物具有降压作用，并在高血压和慢性肾功能衰竭的动物模型中显示出肾脏保护作用。然而，运动训练的这些影响的调节机制仍然不清楚。本研究观察了运动训练对自发性高血压大鼠(SHR)和血压正常的Wistar-京都(WKY)大鼠肾脏一氧化氮合酶(NOS)的影响。将雄性SHR和WKY大鼠随机分为安静组和跑台运动组，连续8周。采用尾套法每隔2周测量一次大鼠的收缩压(SBP)，运动结束后采集尿样和血样。检测肾组织一氧化氮合酶活性、蛋白表达及内皮(E)一氧化氮合酶磷酸化。运动训练显著降低SHR的SBP，减少尿白蛋白排泄，降低硫代巴比妥酸反应物质水平和肾脏NADPH氧化酶活性，增加肌酐清除率。运动训练显著增加SHR和WKY大鼠血、尿硝酸盐/亚硝酸盐、肾组织一氧化氮合酶活性和eNOS及神经元型一氧化氮合酶的表达，但降低肾组织eNOS Ser1177和Thr495的磷酸化水平。肾脏一氧化氮合酶可能参与运动训练对SHR的降压和肾脏保护作用。
Exercise training is known to have antihypertensive effects in humans and animals with hypertension, as well as to exhibit renal protective effects in animal models of hypertension and chronic renal failure. However, the mechanisms regulating these effects of exercise training remain unclear. The present study examined the effects of exercise training on nitric oxide synthase (NOS) in the kidneys of spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto (WKY) rats. Male SHR and WKY rats were randomly divided into a sedentary group and a treadmill exercise group for 8weeks. Systolic blood pressure (SBP) was measured every 2weeks by the tail-cuff method and urine and blood samples were collected after the exercise protocol. Nitric oxide synthase activity and protein expression and endothelial (e) NOS phosphorylation in the kidney were examined. Exercise training significantly lowered SBP, decreased urinary albumin excretion, thiobarbituric acid-reactive substances levels and renal NADPH oxidase activity, and increased creatinine clearance in SHR. Exercise training significantly increased plasma and urinary nitrate/nitrite, NOS activity and eNOS and neuronal NOS expression, but decreased eNOS phosphorylation at Ser1177 and Thr495 in kidneys of SHR and WKY rats. Renal NOS may be involved in the antihypertensive and renal protective effects of exercise training in SHR.