Long-range temporal coordination of gene expression in synthetic microbial consortia
Long-range temporal coordination of gene expression in synthetic microbial consortia
复制标题
合成微生物菌群中基因表达的长距离时间协调
DOI：
10.1038/s41589-019-0372-9
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发表时间：
2019-11-01
影响因子：
14.8
通讯作者：
Bennett, Matthew R.
中科院分区：
文献类型：
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作者：
Kim, Jae Kyoung;Chen, Ye;Bennett, Matthew R.
合成微生物联合体比同基因合成微生物具有优势，因为它们可以在菌株之间分配生化和调节任务。然而，由于信号分子的范围受到扩散的限制，在空间扩展的联合体中很难协调基因的表达。在这里，我们表明，即使当联合体的空间范围远远大于信号分子的扩散距离时，也可以实现基因表达的时空协调。为了做到这一点，我们研究了一个双菌株合成微生物联合体的动力学，它在小菌落中产生相干振荡。在大群体中，我们发现整个种群的时间协调振荡依赖于放大和传播细胞间信号的内在正反馈环的存在。这些结果表明，合成的多细胞系统可以被设计成仅使用组成细胞之间的瞬时、短程耦合来展示协调的基因表达。
Synthetic microbial consortia have an advantage over isogenic synthetic microbes because they can apportion biochemical and regulatory tasks among the strains. However, it is difficult to coordinate gene expression in spatially extended consortia because the range of signaling molecules is limited by diffusion. Here, we show that spatio-temporal coordination of gene expression can be achieved even when the spatial extent of the consortium is much greater than the diffusion distance of the signaling molecules. To do this, we examined the dynamics of a two-strain synthetic microbial consortium that generates coherent oscillations in small colonies. In large colonies, we find that temporally coordinated oscillations across the population depend on the presence of an intrinsic positive feedback loop that amplifies and propagates intercellular signals. These results demonstrate that synthetic multicellular systems can be engineered to exhibit coordinated gene expression using only transient, short-range coupling among constituent cells.