Differential effects of age on circulating and splenic leukocyte populations in C57BL/6 and BALB/c male mice.
Differential effects of age on circulating and splenic leukocyte populations in C57BL/6 and BALB/c male mice.
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DOI：
10.1186/1742-4933-5-1
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发表时间：
2008-02-11
期刊：
影响因子：
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通讯作者：
Filipov NM
中科院分区：
文献类型：
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作者：
Pinchuk LM;Filipov NM
尽管有几份关于免疫系统细胞的年龄相关表型变化的报告，但仍然缺乏使用原型Th 1型和Th 2型极化小鼠品系的特异性和先天免疫细胞群体之间的多点年龄比较的研究。使用多点年龄比较的方法，从两个主要的免疫系统隔室，外周血和脾脏，和流式细胞术分析的细胞，我们发现了几个主要的差异T细胞和专业的抗原呈递细胞（APC）的人口起源于一个典型的辅助性T细胞（Th）1小鼠株，C57 BL/6，和一个典型的Th 2株，BALB/c。例如，无论年龄如何，外周血单核细胞（PBMC）和脾脏中CD 4+（BALB/c品系中较高）、CD 8 + T细胞和CD 11b +/CD 11 c + APC（C57 BL/6小鼠中较高）的比例存在品系差异。其他差异仅存在于PBMC中（C57 BL/6小鼠中II类MHC+和CD 19+更大），或者差异在脾脏中明显，但在循环中不明显（C57 BL/6小鼠中CD 3 + T细胞更大）。两种菌株的PBMC和脾脏中存在随年龄增加的细胞群（MHC II类+），两种菌株的外周和脾脏中存在随年龄减少的细胞群（CD 11b+），或者两种菌株的PBMC和脾脏中存在随年龄增加的细胞群（CD 8+）。我们还发现了幼稚和记忆/活化脾T细胞分布的品系和年龄差异，例如，BALB/c小鼠和C57 BL/6小鼠具有更多的记忆/活化和更少的幼稚CD 8+和CD 4 + T细胞。我们的数据提供了重要的信息的主要差异，在年龄的背景下，在T细胞和专业APC人群之间的原型Th 1小鼠品系C57 BL/6和原型Th 2品系BALB/c。虽然发生的与年龄有关的变化可能相当微妙，但它们可能与疾病和压力的条件非常相关。重要的是，我们的数据表明，在选择合适的小鼠模型进行免疫学研究时，应一致考虑年龄和品系。
Despite several reports on age-related phenotypic changes of the immune system's cells, studies that use a multipoint age comparison between the specific and innate immune cell populations of prototypical Th1- and Th2-type polarized mouse strains are still lacking. Using a multipoint age comparison approach, cells from the two major immune system compartments, peripheral blood and spleen, and flow cytometry analysis, we found several principal differences in T cell and professional antigen presenting cell (APC) populations originating from a prototypical T helper (Th) 1 mouse strain, C57BL/6, and a prototypical Th2 strain, BALB/c. For example, regardless of age, there were strain differences in both peripheral blood mononuclear cells (PBMC) and spleens in the proportion of CD4+ (higher in the BALB/c strain), CD8+ T cells and CD11b+/CD11c+ APC (greater in C57BL/6 mice). Other differences were present only in PBMC (MHC class II + and CD19+ were greater in C57BL/6 mice) or differences were evident in the spleens but not in circulation (CD3+ T cells were greater in C57BL/6 mice). There were populations of cells that increased with age in PBMC and spleens of both strains (MHC class II+), decreased in the periphery and spleens of both strains (CD11b+) or did not change in the PBMC and spleens of both strains (CD8+). We also found strain and age differences in the distribution of naïve and memory/activated splenic T cells, e.g., BALB/c mice had more memory/activated and less naive CD8+ and CD4+ T cells and the C57BL/6 mice. Our data provide important information on the principal differences, within the context of age, in T cell and professional APC populations between the prototypical Th1 mouse strain C57BL/6 and the prototypical Th2 strain BALB/c. Although the age-related changes that occur may be rather subtle, they may be very relevant in conditions of disease and stress. Importantly, our data indicate that age and strain should be considered in concert in the selection of appropriate mouse models for immunological research.