Recombinant T2 RNase protein of Schistosoma japonicum inhibits expression of alpha-SMA in LX-2 cells
Recombinant T2 RNase protein of Schistosoma japonicum inhibits expression of alpha-SMA in LX-2 cells
复制标题
日本血吸虫重组T2 RNase蛋白抑制LX-2细胞α-SMA表达
DOI：
10.1007/s00436-016-5178-z
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发表时间：
2016
影响因子：
2
通讯作者：
Duan Yinong
中科院分区：
文献类型：
--
作者：
Wang Jianxin;Peng Wenxia;Feng Jinrong;Zhu D;an;Chen Jinling;Sun Xiaolei;Lyu Lei;Ju Shaoqing;Duan Yinong
Recombinant T2 RNase glycoprotein, which showed a certain degree of homology to Omega-1 fromSchistosoma mansonieggs, was expressed in adult worms ofSchistosoma japonicum, but not in eggs ofS. japonicum. The direct biological role of the recombinant T2 RNase protein in activation of hepatic stellate cells (HSCs) remains unknown. In the present study, the immortalized human HSC line (LX-2 cells) was treated with the recombinant T2 RNase protein at indicated concentrations for various time points in vitro. The expression levels of α-smooth muscle actin (α-SMA) and Smad4 were detected by Western blot. The results showed that the recombinant T2 RNase protein significantly diminished the expression levels of α-SMA and Smad4 in LX-2 cells. The upregulated expression levels of α-SMA and Smad4 by TGF-β1 in LX-2 cells were both suppressed by the recombinant T2 RNase protein. These data suggest that the recombinant T2 RNase protein may be a potential target of therapeutic strategy for the treatment of hepatic fibrosis.
