Ectopic expression of a cyanobacterial flavodoxin in creeping bentgrass impacts plant development and confers broad abiotic stress tolerance.
Ectopic expression of a cyanobacterial flavodoxin in creeping bentgrass impacts plant development and confers broad abiotic stress tolerance.
复制标题
DOI：
10.1111/pbi.12638
复制
发表时间：
2017-04
影响因子：
13.8
通讯作者：
Luo H
中科院分区：
文献类型：
--
作者：
Li Z;Yuan S;Jia H;Gao F;Zhou M;Yuan N;Wu P;Hu Q;Sun D;Luo H
黄素氧还蛋白（Fld）是光合微生物在逆境条件下的一种重要电子传递蛋白。蓝藻Fld已被证明能够在胁迫条件下的大多数电子传递过程中替代高等植物的铁氧还蛋白。我们已经探索了Fld用于改善匍匐翦股颖（Agrostis stolonifera L.）的植物胁迫反应的潜力。Fld的过量表达改变了植物的生长发育。最重要的是，转基因植物在氧化、干旱和热胁迫以及氮（N）饥饿下表现出显著增强的性能，这与比野生型对照更高的保水性和细胞膜完整性、热休克蛋白基因的修饰表达、更多还原的硫氧还蛋白的产生、氮积累和总叶绿素含量增加以及亚硝酸还原酶和氮转运蛋白基因表达上调。进一步的分析显示，其他应激相关基因的表达也在Fld表达转基因中受到影响。我们的数据建立了关键作用的Fld在调节植物的生长和发育和植物对多种来源的不利环境条件的作物物种的反应。这证明了在作物物种中操纵Fld用于植物耐逆基因工程的可行性。
Flavodoxin (Fld) plays a pivotal role in photosynthetic microorganisms as an alternative electron carrier flavoprotein under adverse environmental conditions. Cyanobacterial Fld has been demonstrated to be able to substitute ferredoxin of higher plants in most electron transfer processes under stressful conditions. We have explored the potential of Fld for use in improving plant stress response in creeping bentgrass (Agrostis stolonifera L.). Overexpression of Fld altered plant growth and development. Most significantly, transgenic plants exhibited drastically enhanced performance under oxidative, drought and heat stress as well as nitrogen (N) starvation, which was associated with higher water retention and cell membrane integrity than wild‐type controls, modified expression of heat‐shock protein genes, production of more reduced thioredoxin, elevated N accumulation and total chlorophyll content as well as up‐regulated expression of nitrite reductase and N transporter genes. Further analysis revealed that the expression of other stress‐related genes was also impacted in Fld‐expressing transgenics. Our data establish a key role of Fld in modulating plant growth and development and plant response to multiple sources of adverse environmental conditions in crop species. This demonstrates the feasibility of manipulating Fld in crop species for genetic engineering of plant stress tolerance.