Allele-specific expression of a variant-specific surface protein (VSP) of Giardia lamblia.
Allele-specific expression of a variant-specific surface protein (VSP) of Giardia lamblia.
复制标题
兰氏贾第鞭毛虫变体特异性表面蛋白 (VSP) 的等位基因特异性表达。
DOI：
10.1093/nar/22.11.2102
复制
发表时间：
1994
影响因子：
14.9
通讯作者：
Adam,RD
中科院分区：
文献类型：
--
作者：
Yang,Y;Adam,RD
贾第鞭毛虫滋养体的表面表现出一组富含半胱氨酸的表面蛋白的可变表达，称为变体特异性表面蛋白（VSP）。克隆的贾第虫素 WBA6 表达 170 kD VSP（VSPA6 或 CRP170），其中包含约 18 至 23 个拷贝的 65 个氨基酸重复序列。我们从基因组文库中克隆了包含 23 个重复的表达的 vspA6 基因，以及从 WBA6 和 WB1269 中克隆了仅具有 8 或 9 个重复的 vspA6 基因的拷贝，WB1269 是源自 WBA6 的克隆系，已丢失了该基因的表达拷贝。含有仅具有8或9个重复的基因的重组克隆在编码区具有8个核苷酸取代。所有重组克隆都映射到相同的染色体位置，但RNA测序和与转录本大小的比较表明，只有具有23个重复的克隆含有产生稳定转录本的基因。对这些数据最可能的解释是G.lamblia滋养体含有vspA6基因的多个等位基因，其中只有一个被表达。
The surfaces ofGiardia lambliatrophozoites demonstrate variable expression of a set of cysteine-rich surface proteins, called variant-specific surface proteins (VSP). The clonedGiardialine, WBA6, expresses a 170 kD VSP (VSPA6 or CRP170) which contains approximately 18 to 23 copies of a 65 amino acid repeat. We have cloned the expressed vspA6 gene containing 23 repeats from a genomic library as well as copies of the vspA6 gene with only 8 or 9 repeats from both WBA6 and from WB1269, a cloned line derived from WBA6 which has lost the expressed copy of the gene. The recombinant clones containing the genes with only 8 or 9 repeats have 8 nucleotide substitutions in the coding region. All the recombinant clones map to the same chromosomal location, yet RNA sequencing and comparison with the transcript size indicate that only the clone with 23 repeats contains a gene producing a stable transcript. The most likely interpretation of these data is thatG.lambliatrophozoites contain multiple alleles of the vspA6 gene of which only one is expressed.