Impact of poxB, pta, and ackA genes on mineral‐weathering of Enterobacter cloacae S71
Impact of poxB, pta, and ackA genes on mineral‐weathering of Enterobacter cloacae S71
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DOI：
10.1002/jobm.201800013
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发表时间：
2018-07
影响因子：
3.1
通讯作者：
Qi Wang;Zhendong Zhang;Ying Zhu;Linyan He;X. Sheng
中科院分区：
文献类型：
--
作者：
Qi Wang;Zhendong Zhang;Ying Zhu;Linyan He;X. Sheng
在这项研究中，比较了矿物风化细菌阴沟肠杆菌S71、通过缺失参与乙酸盐形成的poxB、pta和ackA基因而产生的突变菌株及其互补菌株之间的黑云母风化行为。与菌株S71相比，突变株ΔpoxB在生长初期和中期的细菌生长量下降，突变株Δpta和ΔackA在生长中期和后期的细菌生长量下降。与菌株S71相比，菌株ΔpoxB在风化初期、菌株Δpta在风化初期或中期、菌株ΔackA在风化中后期和整个风化过程中，溶解态Al和Fe浓度均较低。菌株ΔpoxB的乙酸盐产量在风化初期受到抑制，菌株Δpta的乙酸盐产量在风化初期和中期受到抑制，菌株ΔackA的乙酸盐产量在整个风化过程中都受到抑制。总体而言，ackA基因表现出更大的影响溶解铁和乙酸浓度比poxB和pta基因。减少细菌生长和较低的溶解铝，铁，和乙酸盐浓度恢复补充菌株。这些结果表明，菌株S71通过产生乙酸促进矿物风化，其中乙酸盐相关基因具有独特的影响。
In this study, biotite weathering behaviors were compared between mineral‐weathering bacteria Enterobacter cloacae S71, mutant strains created by the deletion of poxB, pta, and ackA genes involved in acetate formation, and their complemented strains. Compared to strain S71, a decrease in bacterial growth was observed during the early and middle stages for the mutant ΔpoxB and at the middle and later stages for the mutants Δpta and ΔackA. Dissolved Al and Fe concentrations were lower during the early stage for strain ΔpoxB, at the early or middle stage for strain Δpta, and at the middle and later stages and throughout the weathering process for strain ΔackA, compared to strain S71. Acetate production was depressed during the early stage for strain ΔpoxB, at the early and middle stages for strain Δpta, and throughout the weathering process for strain ΔackA. Overall, the ackA gene exhibited a larger impact on dissolved Fe and acetate concentrations than both the poxB and pta genes. Reduced bacterial growth and lower dissolved Al, Fe, and acetate concentrations recovered by the complemented strains. These results show that strain S71 promoted mineral weathering through the production of acetic acid with distinctive impacts by the genes involved in acetate.