Effect of chain density and conformation on protein adsorption at PEG-grafted polyurethane surfaces
Effect of chain density and conformation on protein adsorption at PEG-grafted polyurethane surfaces
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链密度和构象对 PEG 接枝聚氨酯表面蛋白质吸附的影响
DOI：
10.1016/j.colsurfb.2007.08.012
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发表时间：
2008-02-15
影响因子：
5.8
通讯作者：
Zhang, Tao
中科院分区：
文献类型：
--
作者：
Chen, Hong;Hu, Xiaoyang;Zhang, Tao
使用一端具有大体积疏水性苄氧基、另一端具有羟基的单苄氧基聚乙二醇（BPEG）作为预构建的BPEG层，以及具有不同链长的聚乙二醇（PEG）和单甲氧基聚乙二醇（MPEG）作为填料对聚氨酯进行改性。我们的目的是研究 PEG 接枝密度和构象对 PEG 化表面蛋白质吸附的影响。通过化学过滤方法估算接枝密度。 ATR-FTIR、AFM 和滴定结果的结合提供了证据，表明可以通过将 PEG 或 MPEG 回填到 BPEG 层来增加接枝密度。然而，在 PEG 或 MPEG 回填后，所有表面上的纤维蛋白原和白蛋白吸附均显着增加。我们得出结论，BPEG 层疏水性苄氧基端基的构象起着关键作用。预构建的 PEG 链的苄氧基端基在 PEG 回填后延伸到表面，这可能是观察到的蛋白质吸附增加的原因。接触角也表明回填 PEG 或 MPEG 后 BPEG 构象发生变化。此外，蛋白质吸附受到填料长度的轻微影响，表明表面形态发生了变化。 (c) 2007 Elsevier B.V. 保留所有权利。
Polyurethanes were modified using monobenzyloxy polyethylene glycol (BPEG) which possesses a bulky hydrophobic benzyloxy group at one end and a hydroxyl group at the other end as a preconstructed BPEG layer, and poly(ethylene glycol) (PEG) and monomethoxyl poly(ethylene glycol) (MPEG) with various chain lengths as fillers. Our objective was to investigate the effect of PEG graft density and conformation on protein adsorption at PEGlated surface. The graft density was estimated by a chemical fitration method. The combination of ATR-FTIR, AFM and titration results provide evidences that the graft density can be increased by backfilling PEG or MPEG to a BPEG layer. However, fibrinogen and albumin adsorption significantly increased on all surfaces after PEG or MPEG backfilling. We conclude that the conformation of hydrophobic benzyloxy end groups of the BPEG layer plays a key role. The benzyloxy end groups of preconstructed PEG chains stretch to the surface after PEG backfilling, which possibly accounts for the observed increase in protein adsorption. The BPEG conformation change after backfilling with PEG or MPEG was also suggested by contact angles. Additionally, protein adsorption was slightly influenced by the length of filler, suggesting a change in surface morphology. (c) 2007 Elsevier B.V. All rights reserved.