SHARPs: Mammalian enhancer-of-split- and hairy-related proteins coupled to neuronal stimulation
SHARPs: Mammalian enhancer-of-split- and hairy-related proteins coupled to neuronal stimulation
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DOI：
10.1006/mcne.1997.0640
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发表时间：
1997-01-01
影响因子：
3.5
通讯作者：
Nave, KA
中科院分区：
文献类型：
--
作者：
Rossner, MJ;Dorr, J;Nave, KA
在哺乳动物中枢神经系统中，一组不同的碱性螺旋-环-螺旋（bHLH）蛋白参与决定祖细胞，随后，在调节神经元分化。在这里，我们报告了一个新的bHLH蛋白亚家族的鉴定，由两个哺乳动物增强分裂和毛相关蛋白，称为SHARP-1和SHARP-2定义。与已知的bHLH基因相反，SHARP基因的可检测转录在胚胎发育结束时开始，标记已达到最终位置的分化神经元，并随着出生后发育的进行而增加。在成人中，SHARP基因在与成人可塑性相关的CNS亚区中表达。在研究神经突生长的模型系统PC 12细胞中，SHARP基因可以被NGF以立即早期基因的动力学诱导。类似地，在体内施用红藻氨酸后1小时内，在整个大鼠大脑皮层的神经元中诱导SHARP-P。这表明，神经元bHLH蛋白也参与了成熟CNS神经元的“适应性”变化，这些变化与神经元兴奋刺激有关。
In the mammalian central nervous system, a diverse group of basic helix-loop-helix (bHLH) proteins is involved in the determination of progenitor cells and, subsequently, in regulating neuronal differentiation. Here we report the identification of a novel subfamily of bHLH proteins, defined by two mammalian enhancer-of-split- and hairy-related proteins, termed SHARP-1 and SHARP-2. In contrast to known bHLH genes, detectable transcription of SHARP genes begins at the end of embryonic development marking differentiated neurons that have reached a final position, and increases as postnatal development proceeds. in the adult, SHARP genes are expressed in subregions of the CNS that have been associated with adult plasticity. In PC12 cells, a model system to study neurite outgrowth, SHARP genes can be induced by NGF with the kinetics of an immediate-early gene. Similarly, within 1 h after the administration of kainic acid in vivo, SHARP-P is induced in neurons throughout the rat cerebral cortex. This suggests that neuronal bHLH proteins are also involved in the ''adaptive'' changes of mature CNS neurons which are coupled to glutamatergic stimulation.