Uterine distension differentially affects remodelling and distensibility of the uterine vasculature in non-pregnant rats.
Uterine distension differentially affects remodelling and distensibility of the uterine vasculature in non-pregnant rats.
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子宫扩张对非妊娠大鼠子宫脉管系统的重塑和扩张有不同的影响。
DOI：
10.1071/rd11208
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Mandalà,Maurizio
中科院分区：
文献类型：
--
作者：
Osol,George;Barron,Carolyn;Mandalà,Maurizio
在妊娠期间，哺乳动物的子宫循环经历了正常妊娠结局所必需的显著扩张性重塑。基本机制定义不清。本研究的目的是通过开发一种新的手术方法来诱导非妊娠大鼠单侧子宫扩张，以验证子宫肌层牵张积极刺激子宫血管重塑的假设。手术后3周，记录了子宫腔、子宫动脉和子宫系膜的长度、直径和扩张性，其中包括将医用级硅胶注入子宫腔。测量桡动脉内径、扩张性和血管平滑肌细胞分裂率（Ki 67染色）。单侧子宫扩张导致子宫主动脉、主静脉和子宫系膜长度显著增加，但对血管直径和扩张性无影响。相反，与扩张的子宫相关的桡动脉直径显著增加。这些变化伴随着动脉扩张性降低和血管肌肉增生增加。总之，这是第一份报告表明，子宫肌层牵拉是一个足够的刺激，以诱导显着的重塑子宫血管在非妊娠大鼠。此外，结果表明这些生长过程的差异调节作为血管的大小和类型的函数。
During pregnancy the mammalian uterine circulation undergoes significant expansive remodelling necessary for normal pregnancy outcome. The underlying mechanisms are poorly defined. The goal of this study was to test the hypothesis that myometrial stretch actively stimulates uterine vascular remodelling by developing a new surgical approach to induce unilateral uterine distension in non-pregnant rats. Three weeks after surgery, which consisted of an infusion of medical-grade silicone into the uterine lumen, main and mesometrial uterine artery and vein length, diameter and distensibility were recorded. Radial artery diameter, distensibility and vascular smooth muscle mitotic rate (Ki67 staining) were also measured. Unilateral uterine distension resulted in significant increases in the length of main uterine artery and vein and mesometrial segments but had no effect on vessel diameter or distensibility. In contrast, there were significant increases in the diameter of the radial arteries associated with the distended uterus. These changes were accompanied by reduced arterial distensibility and increased vascular muscle hyperplasia. In summary, this is the first report to show that myometrial stretch is a sufficient stimulus to induce significant remodelling of uterine vessels in non-pregnant rats. Moreover, the results indicate differential regulation of these growth processes as a function of vessel size and type.