Cytological characterization of a thermo-sensitive cytoplasmic male-sterile wheat line having K-type cytoplasm of Aegilops kotschyi.
Cytological characterization of a thermo-sensitive cytoplasmic male-sterile wheat line having K-type cytoplasm of Aegilops kotschyi.
复制标题
热敏感的细胞质雄性小麦的细胞学表征，具有aegilops kotschyi的K型细胞质。
DOI：
10.1270/jsbbs.16039
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发表时间：
2016-12
期刊：
影响因子：
2.4
通讯作者：
Song X
中科院分区：
文献类型：
--
作者：
Meng L;Liu Z;Zhang L;Hu G;Song X
雄性不育是获得作物杂种优势的重要手段。利用基于分蘖再生的新方法育成了温敏型细胞质雄性不育系。在本研究中，我们探索了保持温敏型雄性不育系的关键生长阶段，发现育性与绒毡层和小孢子的异常发育有关。我们研究了不同发育阶段经温度处理的植物花药的育性和细胞学。温敏雄性不育系KTM3315A在Zadoks生育期41-49和58-59表现温敏型雄性不育。在形态上，该品系在抽穗前3~9d和开花前3~6d表现温敏型雄性不育，春夏两季有3个地点部分恢复。光温敏核不育系KTM3315A植株从小孢子发育的单核早期开始，直到绒毡层细胞完全退化，即发生绒毡层细胞程序性死亡(PCD)。小孢子发育受阻，败育类型为染色败育。因此，KTM3315A雄性不育系的育性对温度敏感，绒毡层早熟PCD是导致花粉败育的主要原因，在一定温度下决定K型雄性不育系的起始期并影响雄性育性转换。
Male sterility is an important tool for obtaining crop heterosis. A thermo-sensitive cytoplasmic male-sterile (TCMS) line was developed recently using a new method based on tiller regeneration. In the present study, we explored the critical growth stages required to maintain thermo-sensitive male sterility in TCMS lines and found that fertility is associated with abnormal tapetal and microspore development. We investigated the fertility and cytology of temperature-treated plant anthers at various developmental stages. TCMS line KTM3315A exhibited thermo-sensitive male sterility in Zadoks growth stages 41–49 and 58–59. Morphologically, the line exhibited thermo-sensitive male sterility at 3–9 days before heading and at 3–6 days before flowering, and it was partially restored in three locations during spring and summer. TCMS line KTM3315A plants exhibited premature tapetal programmed cell death (PCD) from the early uninucleate stage of microspore development until the tapetal cells degraded completely. Microspore development was then blocked and the pollen abortion type was stainable abortion. Thus, male fertility in the line KTM3315A is sensitive to temperature and premature tapetal PCD is the main cause of pollen abortion, where it determines the starting period and affects male fertility conversion in K-type TCMS lines at certain temperatures.