Human herpesvirus 6A induces apoptosis of HSB-2 cells via a mitochondrion-related caspase pathway.
Human herpesvirus 6A induces apoptosis of HSB-2 cells via a mitochondrion-related caspase pathway.
复制标题
人疱疹病毒 6A 通过线粒体相关的 caspase 途径诱导 HSB-2 细胞凋亡
DOI：
10.1016/s1674-8301(10)60059-0
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发表时间：
2010-11
影响因子：
2.3
通讯作者：
Yao, Kun
中科院分区：
文献类型：
--
作者：
Li, Lingyun;Chi, Jing;Zhou, Feng;Guo, Dandan;Wang, Fang;Liu, Genyan;Zhang, Chun;Yao, Kun
细胞凋亡在病毒感染的发病机制中起着重要作用。在这项研究中，我们研究了生产性 HHV-6A 感染过程中的细胞死亡过程及其潜在机制。 annexin V-PI染色和电镜表明HHV-6A是细胞凋亡的强诱导剂。 HHV-6A感染降低了线粒体跨膜电位并导致线粒体形态变化。细胞死亡与 caspase-3 的激活和 DNA 修复酶聚（ADP-核糖）聚合酶的裂解有关，已知聚（ADP-核糖）聚合酶是激活 caspase-3 的重要底物。 Caspase-9在HHV-6A感染的细胞中显着激活，而caspase-8则不明显激活。此外，HHV-6A感染上调Bax并下调Bcl-2。这是 HHV-6A 感染细胞中线粒体介导的 caspase 依赖性细胞凋亡的首次证明。
Apoptosis plays an important role in the pathogenesis of viral infections. In this study, we investigated the cell death processes during productive HHV-6A infection and the underlying mechanisms. Annexin V-PI staining and electron microscopy indicated that HHV-6A is a strong inducer of apoptosis. HHV-6A infection decreased mitochondrial transmembrane potential and led to morphological changes of mitochondria. The cell death was associated with activation of caspase-3 and cleavage of DNA repair enzyme poly (ADP-ribose) polymerase, which is known to be an important substrate for activated caspase-3. Caspase-9 was activated significantly in HHV-6A-infected cells, whereas caspase-8 was not activated obviously. Moreover, HHV-6A infection upregulated Bax and downregulated Bcl-2. This is the first demonstration of mitochondrion-mediated, caspase-dependent apoptosis in HHV-6A-infected cells.