Inactivation and Survival of Hepatitis C Virus on Inanimate Surfaces
Inactivation and Survival of Hepatitis C Virus on Inanimate Surfaces
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DOI：
10.1093/infdis/jir535
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发表时间：
2011-12-15
影响因子：
6.4
通讯作者：
Steinmann, Eike
中科院分区：
文献类型：
--
作者：
Doerrbecker, Juliane;Friesland, Martina;Steinmann, Eike
背景来自无生命表面或物体的丙型肝炎病毒（HCV）交叉污染与卫生保健机构和注射吸毒者中的HCV传播有关。我们建立了基于HCV的载体和药物传播试验，模拟实际条件，研究HCV在无生命表面上的灭活和存活。使用真实细胞培养物衍生的病毒进行研究。将HCV在钢盘上干燥，并测试杀生物剂对HCV的杀病毒功效。通过有限稀释测定法测定亲和性。HCV的稳定性在载体试验中分析了几天，或在药物传播试验中使用勺子作为炊具。HCV可以在载体测定中有效地干燥和回收。最有效的酒精消毒病毒是1-丙醇，市售的消毒剂降低了HCV的感染性到检测不到的水平。无生命表面上的病毒感染性在血清存在下可检测长达5天，并且需要约65 - 70摄氏度的温度来消除药物传播测定中的感染性。这些发现对于评估HCV传播风险非常重要，并应有助于制定严格的公共卫生干预措施以预防HCV感染。
Background. Hepatitis C virus (HCV) cross-contamination from inanimate surfaces or objects has been implicated in transmission of HCV in health-care settings and among injection drug users. We established HCV-based carrier and drug transmission assays that simulate practical conditions to study inactivation and survival of HCV on inanimate surfaces.Methods. Studies were performed with authentic cell culture derived viruses. HCV was dried on steel discs and biocides were tested for their virucidal efficacy against HCV. Infectivity was determined by a limiting dilution assay. HCV stability was analyzed in a carrier assay for several days or in a drug transmission assay using a spoon as cooker.Results. HCV can be dried and recovered efficiently in the carrier assay. The most effective alcohol to inactivate the virus was 1-propanol, and commercially available disinfectants reduced infectivity of HCV to undetectable levels. Viral infectivity on inanimate surfaces was detectable in the presence of serum for up to 5 days, and temperatures of about 65-70 degrees C were required to eliminate infectivity in the drug transmission assay.Conclusions. These findings are important for assessment of HCV transmission risks and should facilitate the definition of stringent public health interventions to prevent HCV infections.