Evidence for probiotic potential of a capsular-producing Streptococcus thermophilus CHCC 3534 strain.
Evidence for probiotic potential of a capsular-producing Streptococcus thermophilus CHCC 3534 strain.
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产荚膜嗜热链球菌 CHCC 3534 菌株益生菌潜力的证据。
DOI：
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发表时间：
2009
影响因子：
2.1
通讯作者：
R. Khalil
中科院分区：
文献类型：
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作者：
R. Khalil
本研究的目的是评估一株被包膜的嗜热链球菌CHCC 3534菌株的益生菌潜力。该菌株耐受0.4%的氧化胆（胆汁），并对低至2.5的pH值暴露3小时具有足够的抵抗力。该菌株对人类肠道粘液具有高度粘附性，对不同抗生素具有独特的耐药性。粗提物。嗜热菌CHCC 3534含有广谱扩散性抗菌化合物“细菌素”，能抑制密切相关的乳酸菌和鼠伤寒沙门菌、金黄色葡萄球菌等多种食品腐败菌的生长。细菌素热稳定，耐pH值，可被蛋白水解酶灭活，抗a-淀粉酶和脂肪酶。对部分纯化的细菌素进行SDS-PAGE分析，发现其中一个组分的分子量在14.4至18.4 kDa之间。该菌株可能具有工业意义，在生物保存、益生菌食品配方和控制食源性病原体引起的腐败方面具有有趣的候选意义。
The purpose of this research was to evaluate the probiotic potential of an capsulated Streptococcus thermophilus CHCC 3534 strain. The strain tolerates 0.4% oxgall (bile) and was sufficiently resistant to pH as low as 2.5 for 3 hours of exposure. The strain demonstrated high adherence to human intestinal mucus, and showed unique resistance to different antibiotics. Crude extracts ofS. thermophilus CHCC 3534 contained a diffusible antimicrobial compound "bacteriocin" with a broad spectrum that inhibited the growth of closely related lactic acid bacteria and a number of food spoilage bacteria including Salmonella typhimurium and Staphylococcus aureus. The bacteriocin was heat stable, resistant to pH, inactivated by proteolytic enzymes, and resistant to a-amylase and lipase. A SDS-PAGE analysis of the partially purified bacteriocin revealed one component with a molecular weight ranging from 14.4 to 18.4 kDa. The strain may have industrial significance and represents an interesting candidate for use in biopreservation, probiotic food formulations and in the control of spoilage caused by food borne pathogens.