Novel electrochemical catalysis as signal amplified strategy for label-free detection of neuron-specific enolase
Novel electrochemical catalysis as signal amplified strategy for label-free detection of neuron-specific enolase
复制标题
新型电化学催化作为信号放大策略用于无标记检测神经元特异性烯醇化酶
DOI：
10.1016/j.bios.2011.10.055
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发表时间：
2012-01-15
影响因子：
12.6
通讯作者：
Yuan, Ruo
中科院分区：
文献类型：
--
作者：
Han, Jing;Zhuo, Ying;Yuan, Ruo
A label-free electrochemical immunoassay for neuron-specific enolase (NSE), a kind of lung cancer marker, was developed in this work via novel electrochemical catalysis for signal amplification. The new amplified strategy was based on the electrochemical catalysis of nickel hexacyanoferrates nanoparticles (NiHCFNPs) in the presence of dopamine (DA). NiHCFNPs, which were assembled on the porous gold nanocrystals (AuNCs) modified glassy carbon electrode (GCE), could exhibit a distinct pair of redox peaks corresponding to anodic and cathodic reactions of hexacyanoferrate (II/III). Subsequently, gold nanoparticles functionalized graphene nanosheets (Au-Gra) were coated on the surface of NiHCFNPs/AuNCs film. Then an enhanced amount of neuron-specific enolase antibody (anti-NSE) could be loaded to obtain a sensitive immunosensor of anti-NSE/Au-Gra/NiHCFNPs/AuNCs/GCE due to the strong adsorption capacity and large specific surface area of Au-Gra. More importantly, the oxidation peak current can be enormously enhanced towards the electrocatalytic oxidation of DA based on NiHCFNPs, resulting in the further improvement of the immunosensor sensitivity. Under optimal conditions, the electrochemical immunosensor exhibited a linear range of 0.001-100 ng/mL with a detection limit of 0.3 pg/mL (S/N = 3). Thus, the proposed immunosensor provides a rapid, simple, and sensitive immunoassay protocol for NSE detection, which may hold a promise for clinical diagnosis. (C) 2011 Elsevier B.V. All rights reserved.
