Association between periodontitis and hyperlipidemia: Cause or effect?
Association between periodontitis and hyperlipidemia: Cause or effect?
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DOI：
10.1902/jop.1999.70.12.1429
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发表时间：
1999-12-01
影响因子：
4.3
通讯作者：
Iacopino, AM
中科院分区：
文献类型：
--
作者：
Cutler, CW;Shinedling, EA;Iacopino, AM
背景：流行病学研究表明牙周炎与冠状动脉疾病之间存在关系，但其机制尚未确定。最近对动物的研究表明，低剂量的内毒素（如革兰氏阴性感染）可诱发高脂血症和骨髓细胞过度活跃。牙周炎、全身暴露于牙龈卟啉单胞菌、脂多糖 (LPS) 和高脂血症之间的关联尚未在人体中进行研究。方法：从 26 名成人牙周炎患者和从患者和工作人员中选出的 25 名健康对照 (C) 受试者中获取血清。分别通过酶联免疫吸附测定（ELISA）和蛋白质印迹分析针对牙龈卟啉单胞菌及其LPS的血清抗体。血清甘油三酯（TG）和胆固醇（CHOL）由商业实验室测定。 AP 与血液中 TG、CHOL 和抗 P 水平之间的关联。通过逻辑回归分析检查牙龈线虫全细胞和LPS。将来自 6 名健康禁食捐献者的外周血多形核白细胞 (PMN) 与纯化的 TG (0.1 mg/ml) 在 37 摄氏度下孵育 2 小时，用 100 ng/ml 牙龈卟啉单胞菌 LPS 刺激，并通过 ELISA 测量 IL-1 β 的释放。 结果：牙周炎的存在与年龄显着相关（比值比 = 3.5，P = 0.04），TG 水平升高（比值比 = 8.6，P = 0.0009），CHOL 水平升高（比值比 = 7，P = 0.004），ELISA 效价升高（比值比 = 35，P = 0.003）以及与牙龈卟啉单胞菌 LPS 的反应性（比值比 = 41，P = 0.001）。当用 100 ng/ml 牙龈卟啉单胞菌 LPS 刺激时，所有 6 名健康患者的 PMN 均释放出中等水平的 IL-1 β（10 至 60 pg/ml）。添加 TG 导致 IL-1 β 分泌显着增加 (P < 0.05)，比单独 LPS 增加 7% 至 150%。 TG 或单独的媒介物均未引发 IL-1 β。结论：本研究的结果表明，牙周炎、高脂血症和针对牙龈卟啉单胞菌 LPS 的血清抗体之间存在显着关系，值得在更大的患者群体中进行进一步检查。此外，这些研究表明，升高的甘油三酯能够调节牙龈卟啉单胞菌 LPS 刺激的 PMN 产生 IL-1 β。
Background: Epidemiological studies suggest a relationship between periodontitis and coronary artery disease, but the mechanism has not been established. Recent studies in animals indicate that low dose endotoxin, as in a Gram-negative infection, can induce hyperlipidemia and myeloid cell hyperactivity. The association between periodontitis, systemic exposure to Porphyromonas gingivalis, lipopolysaccharides (LPS), and hyperlipidemia has not been examined in humans.Methods: Sera were obtained from 26 adult periodontitis patients and 25 healthy control (C) subjects selected from patients and staff. Serum antibodies against Porphyromonas gingivalis and its LPS were analyzed by enzyme-linked immunosorbent assay (ELISA) and Western blotting, respectively. Serum triglycerides (TG) and cholesterol (CHOL) were assayed by a commercial laboratory. The associations between AP and blood levels of TG, CHOL, and anti-P. gingivalis whole cells and LPS were examined by logistic regression analysis. Peripheral blood polymorphonuclear leukocytes (PMNs) from 6 healthy fasted donors were incubated with purified TG (0.1 mg/ml) for 2 hours at 37 degrees C, stimulated with 100 ng/ml P. gingivalis LPS, and the release of IL-1 beta measured by ELISA.Results: The presence of periodontitis was significantly associated with age (odds ratio = 3.5, P = 0.04), elevated TG levels (odds ratio = 8.6, P = 0.0009), elevated CHOL levels (odds ratio = 7, P = 0.004), elevated ELISA titer (odds ratio = 35, P = 0.003) and reactivity with P. gingivalis LPS (odds ratio = 41, P = 0.001). PMNs from all 6 healthy patients released modest levels of IL-1 beta (10 to 60 pg/ml) when stimulated with 100 ng/ml P. gingivalis LPS. Addition of TG resulted in a significant increase (P < 0.05) in IL-1 beta secreted that ranged from 7 to 150% over LPS alone. No IL-1 beta was elicited by TG or vehicle alone.Conclusions: The results of this study indicate the presence of a significant relationship between periodontitis, hyperlipidemia, and serum antibodies against P. gingivalis LPS that warrants further examination in a larger patient population. Furthermore, these studies indicate that elevated triglycerides are able to modulate IL-1 beta production by PMNs stimulated with P. gingivalis LPS.