Tissue microarrays as a platform for proteomic investigation.
Tissue microarrays as a platform for proteomic investigation.
复制标题
组织微阵列作为蛋白质组研究的平台。
DOI：
10.1007/s10735-006-9049-2
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发表时间：
2007
影响因子：
3.2
通讯作者：
Hewitt,StephenM
中科院分区：
文献类型：
--
作者：
Chung,Joon-Yong;Braunschweig,Till;Tuttle,Kimberly;Hewitt,StephenM
组织微阵列已成为转化病理学的重要工具。它们用于确认来自其他实验平台的结果，例如表达微阵列，以及通过免疫组织化学分析探索蛋白质表达谱的主要工具。组织微阵列通常用于分子流行病学、药物开发和确定新生物标志物的诊断、预后和预测价值。通过将传统的基于蛋白质的测定以及新的测定应用于该平台，组织微阵列已经获得了作为基础科学以及临床研究的蛋白质组学工具的新效用。本文将探讨新的方法，正在应用于组织微阵列，表征人类蛋白质组，和新技术，使组织微阵列作为一个蛋白质阵列的功能。
Tissue microarrays have become an essential tool in translational pathology. They are used to confirm results from other experimental platforms, such as expression microarrays, as well as a primary tool to explore the expression profile of proteins by immunohistochemical analysis. Tissue microarrays are routinely used molecular epidemiology, drug development and determining the diagnostic, prognostic and predictive value of new biomarkers. By applying traditional protein based assays, as well as novel assays to the platform, tissue microarrays have gained a new utility as a proteomic tool for both basic science as well as clinical investigation. This article will explore the new approaches that are being applied to tissue microarrays to, characterize the human proteome, and new technologies that allow tissue microarrays to function as a protein array.