Expression of two methionine-rich storage protein genes of Plutella xylostella (L.) in response to development, juvenile hormone-analog and pyrethroid
Expression of two methionine-rich storage protein genes of Plutella xylostella (L.) in response to development, juvenile hormone-analog and pyrethroid
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DOI：
10.1016/j.cbpb.2007.04.017
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发表时间：
2007-09-01
影响因子：
2.2
通讯作者：
Tsumuki, Hisaaki
中科院分区：
文献类型：
--
作者：
Ashfaq, Muhammad;Sonoda, Shoji;Tsumuki, Hisaaki
本研究克隆并鉴定了小菜蛾贮藏蛋白基因PxSP1和PxSP2的基因序列，并对其表达进行了研究。PxSP1和PxSP2分别编码91 kDa的蛋白。两个基因的核苷酸同源性和氨基酸同源性分别为79%和82%。氨基酸组成(蛋氨酸和GT；4%)、与其他昆虫贮藏蛋白的序列同源性和系统发育分析表明，这些基因属于中等蛋氨酸含量的SP基因亚家族。这些基因主要在末龄雌幼虫中表达，用保幼激素类似物处理后，其mRNA水平被抑制。用亚致死剂量的拟除虫菊酯处理雌幼虫，可显著提高这两个基因的mRNA水平。拟除虫菊酯对PxSP1和PxSP2基因的诱导可能与生殖有关，因为蛋氨酸丰富的蛋白质是已知的鸡蛋生产的关键因素。(C)2007 Elsevier Inc.保留所有权利。
We cloned and characterized cDNA of two storage protein (SP) genes, PxSP1 and PxSP2, from the diamondback moth, Plutella xylostella (Lepidoptera: Yponomeutidae) and investigated their expression. PxSP1 and PxSP2 each encoded a putative protein of 91 kDa. Nucleotide and deduced amino acid identities between the two genes were 79% and 82%, respectively. Amino acid composition (methionine >4%), sequence homology with other insect storage proteins and the phylogenetic analysis suggested that the genes belong to the subfamily of moderately methionine-rich SP genes. The genes were predominantly expressed in the last instar female larvae and the mRNA levels were suppressed by treatment with a juvenile hormone-analog. Treatment of female larvae with sublethal dose of a pyrethroid caused a significant increase in mRNA levels of both genes. Induction of PxSP1 and PxSP2 genes as a result of pyrethroid application may have implications with respect to reproduction as methionine-rich proteins are known as a key element for egg production. (C) 2007 Elsevier Inc. All rights reserved.