VASCULARIZATION OF THE CONTINUOUS HUMAN COLONIC-CANCER CELL-LINE LS-174-T DEPOSITED SUBCUTANEOUSLY IN NUDE RATS
VASCULARIZATION OF THE CONTINUOUS HUMAN COLONIC-CANCER CELL-LINE LS-174-T DEPOSITED SUBCUTANEOUSLY IN NUDE RATS
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DOI：
10.1111/j.1699-0463.1988.tb00933.x
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发表时间：
1988-08-01
期刊：
影响因子：
2.8
通讯作者：
LORELIUS, LE
中科院分区：
文献类型：
--
作者：
AHLSTROM, H;CHRISTOFFERSON, R;LORELIUS, LE
通过光学显微镜、血管造影术和微血管腐蚀铸型以及扫描电子显微镜分析，研究了在先天性无胸腺大鼠的两条后腿中皮下沉积后2-8周的人结肠癌细胞系LS 174 T的大血管和微血管。肿瘤血管与股动脉分支相连。仅肿瘤的外部200-500 μ m广泛血管化，具有几个同心的、不完整的曲折血管层，类似于洋葱皮。光学显微镜检查显示肿瘤中心有坏死和出血，尤其是在较老的肿瘤中，这与在管型中观察到的中心无血管性很好地相符。在邻近的肌筋膜中，微静脉和毛细血管密度增加，并向肿瘤方向弯曲。可以得出结论，细胞系LS 174 T生长侵入性向内，并从裸大鼠宿主招募其血管。总体肿瘤血管模式是无组织的，表明对新血管形成的控制有限。树脂外渗，这是在所有铸件肿瘤中遇到的，可以是血管通透性增强的一个粗略指标。
The macro- and microvasculature of the human colonic cancer cell line LS 174 T, 2-8 weeks after subcutaneous deposition in both hind legs of congenitally athymic rats was investigated by light microscopy, angiography, and microvascular corrosion casting with analysis in a scanning electron microscope. The tumour blood vessels were connected to branches of the femoral artery. Only the outer 200-500 .mu.m of the tumour was extensively vascularized, with several concentric, incomplete layers of tortuous vessels, resembling onion skin. Light microscopy revealed necrosis and bleeding in the centre of the tumour, especially in the older tumours, which corresponded well to the central avascularity observed in the casts. There was an increase in venular and capillary density and tortousity towards the tumour in the adjacent muscular fascia. It is concluded that the cell line LS 174 T grows invasively inwards and recruits its vessels from the nude rat host. The overall tumour vascular pattern was unorganized, suggesting limited control of new vessel formation. Extravasations of resin, which were encountered in all cast tumors, can be a rough indicator of enhanced vascular permeability.