STUDIES OF RELEASE FROM HUMAN PLATELETS OF GROWTH-FACTOR FOR CULTURED HUMAN ARTERIAL SMOOTH-MUSCLE CELLS
STUDIES OF RELEASE FROM HUMAN PLATELETS OF GROWTH-FACTOR FOR CULTURED HUMAN ARTERIAL SMOOTH-MUSCLE CELLS
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DOI：
10.1161/01.res.42.3.402
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发表时间：
1978-01-01
影响因子：
20.1
通讯作者：
GOODMAN, DS
中科院分区：
文献类型：
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作者：
WITTE, LD;KAPLAN, KL;GOODMAN, DS
血小板含有促进动脉平滑肌细胞（SMC）生长的因子，SMC可能在动脉粥样硬化形成中起主要作用。本文研究了血小板源性生长因子（PDGF）对培养的人动脉平滑肌细胞的某些作用，以及血小板释放PDGF与其它释放物质的关系。从血小板释放的物质在刺激人SMC增殖中是高度有效的。人血清的促生长活性的很大一部分可归因于从血小板释放的因子。在人SMC和小鼠3 T3细胞中观察到了与PDGF相似的剂量反应模式。与血清素、ADP、ATP、酸水解酶、血小板因子4（PF 4）和β-凝血酶相比，测定PDGF释放的时间过程及其对人凝血酶的浓度依赖性。血小板球蛋白（β TG）。通过刺激3 H-胸苷掺入到3 T3细胞的DNA中来测定PDGF活性;通过新开发的放射免疫测定来测量PF 4和β TG。通过比释放其它组分所需的凝血酶浓度低的凝血酶浓度，PDGF、PF 4和β TG从血小板中释放。显然，PDGF、PF 4和β TG以不同于致密体（其含有5-羟色胺、ADP、ATP）或含有酸性水解酶的颗粒的颗粒定位在血小板中，可能定位在α-TG中。颗粒。这些含PDGF的α-颗粒在释放反应期间主动释放，并且对低剂量凝血酶的释放特别敏感。
Platelets contain a growth-promoting factor for arterial smooth muscle cells (SMC) that may play a major role in atherogenesis. Some of the effects of the platelet-derived growth factor (PDGF) on human arterial SMC in culture and the release of PDGF from human platelets in relationship to other released substances were studied. Material released from platelets was highly potent in stimulating human SMC to proliferate. A substantial portion of the growth-promoting activity of human serum could be attributed to a factor(s) released from platelets. Similar dose-response patterns to PDGF were observed with human SMC and with mouse 3T3 cells. The time-course of release of PDGF and its concentration dependence on human thrombin were determined in comparison with serotonin, ADP, ATP, an acid hydrolase, platelet factor 4 (PF4) and .beta.-thromboglobulin (.beta.TG). PDGF activity was assay by stimulation of the incorporation of 3H-thymidine into DNA of 3T3 cells; PF4 and .beta.TG were measured by newly developed radioimmunoassays. PDGF, PF4 and .beta.TG were released from platelets by lower concentrations of thrombin than those required for release of the other components. Apparently PDGF, PF4 and .beta.TG are localized in the platelet in granules different from either the dense bodies (that contain serotonin, ADP, ATP) or the acid hydrolase-containing granules, possibly in .alpha.-granules. The contents of these PDGF-containing .alpha.-granules are actively released during the release reaction and are particularly sensitive to release by low doses of thrombin.